Mouse recombinant CXCL5 (C-X-C motif chemokine 5) protein, AF

About this product

C-X-C motif chemokine 5 (CXCL5) also named epithelial-derived neutrophil-activating peptide 78 (ENA-78), which is a chemokine of the intercrine alpha family. CXCL5 is a 8.2 kDa protein containing 74 amino acid residues. CXCL5 is stimulated by the IL-1 or TNFα during inflammation which produced by the eosinophils and CXCL5 is inhibited by the IFNγ.CXCL5 promotes the formation of blood vessels and angiogenesis by binding the cell receptor CXCR2.

Specifications

Catalog number

PROTP50228-4-20ug

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Epithelial Neutrophil Activating Peptide-78, ENA-78, AMCF, AMCF-II, Cxcl, Cxcl6, GCP-, GCP-2, L, LIX, Scyb, Scyb5, Scyb6

Gene Name

CXCL5

UniProt

P50228

Reactivity

Mouse

Tag

His Tag (N-term)

Source

Escherichia coli

Applications

Cell Culture

Purification

>98% as determined by SDS-PAGE.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Bioactivity

Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2. The ED50 for this effect is <100 ng/mL.

Form

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Molecular Weight

The protein has a calculated MW of 10.62 kDa. The protein migrates below 15-17 kDa under reducing condition (SDS-PAGE analysis) .

Storage Conditions

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.

AA Sequence

VIAATELRCVCLTVTPKINPKLIANLEVIPAGPQCPTVEVIAKLKNQKEVCLDPEAPVIKKIIQKILGSDKKKA with polyhistidine tag at the N-terminus.