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Product details
Catalog number #10405
Price for #10405
Size / count 1x 100 µL
NucView® 405 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView® caspase-3 substrates can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. NucView® substrates consist of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with fluorescence. Thus, NucView caspase-3 substrates are bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The staining is also formaldehyde-fixable. NucView® 405 dye is excited by the 405 nm laser line for detection by confocal microscopy in the DAPI channel, or by flow cytometry in the Pacific Blue® channel. NucView® 405 is ideal for caspase-3 detection in multi-color applications for researchers who wish to reserve the green fluorescence channel for other detection reagents. Note: NucView® 405 signal may be difficult to observe using epifluorescence microscopy. Confocal microscopy using 405 nm laser excitation is recommended for imaging. NucView® 405 also is available at 1 mM in PBS for use in DMSO-sensitive cells. Biotium also offers green fluorescent NucView® 488 and orange fluorescent NucView® caspase-3 substrates. Pacific Blue is a registered trademark of Thermo Fisher Scientific.
The catalog number for NucView® 405 Caspase-3 Substrate, 1 mM in DMSO is #10405.
NucView® 405 Caspase-3 Substrate, 1 mM in DMSO is available in the following size(s): 1x 100 µL.
NucView® 405 Caspase-3 Substrate, 1 mM in DMSO is currently made to order / not held in immediate stock. Contact us for lead time.
NucView® 405 Caspase-3 Substrate, 1 mM in DMSO is manufactured by Biotium.
Estimated delivery time for NucView® 405 Caspase-3 Substrate, 1 mM in DMSO is approximately 15 business day(s), depending on your location.
The safety data sheet for NucView® 405 Caspase-3 Substrate, 1 mM in DMSO is available here: https://cdn.gentaur.com/products/37/699c39d20d8c9d341e16462e/msds/%2310405.pdf.
NucView® 405 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA)
The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.
As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.
Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.
