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Product details
Catalog number #10407-T
Price for #10407-T
Size / count 1x 10 µL
NucView® 405 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView® caspase-3 substrates can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. NucView® substrates consist of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with fluorescence. Thus, NucView caspase-3 substrates are bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The staining is also formaldehyde-fixable. NucView® 405 dye is excited by the 405 nm laser line for detection by confocal microscopy in the DAPI channel, or by flow cytometry in the Pacific Blue® channel. NucView® 405 is ideal for caspase-3 detection in multi-color applications for researchers who wish to reserve the green fluorescence channel for other detection reagents. Note: NucView® 405 signal may be difficult to observe using epifluorescence microscopy. Confocal microscopy using 405 nm laser excitation is recommended for imaging. NucView® 405 Caspase-3 Substrate also is offered as a 1 mM solution in DMSO. The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity. In non-DMSO sensitive cell types, adding DMSO during the substrate incubation may enhance NucView® 405 staining. Biotium also offers green fluorescent NucView® 488 caspase-3 substrate and kits, as well as orange fluorescent NucView® 530. Pacific Blue is a registered trademark of Thermo Fisher Scientific.
The catalog number for NucView® 405 Caspase-3 Substrate, 1 mM in 1X PBS, Trial Size is #10407-T.
NucView® 405 Caspase-3 Substrate, 1 mM in 1X PBS, Trial Size is available in the following size(s): 1x 10 µL.
NucView® 405 Caspase-3 Substrate, 1 mM in 1X PBS, Trial Size is currently made to order / not held in immediate stock. Contact us for lead time.
NucView® 405 Caspase-3 Substrate, 1 mM in 1X PBS, Trial Size is manufactured by Biotium.
Estimated delivery time for NucView® 405 Caspase-3 Substrate, 1 mM in 1X PBS, Trial Size is approximately 15 business day(s), depending on your location.
The safety data sheet for NucView® 405 Caspase-3 Substrate, 1 mM in 1X PBS, Trial Size is available here: https://cdn.gentaur.com/products/37/699c39d20d8c9d341e164633/msds/%2310407-t.pdf.
NucView® 405 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) a
Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.
Yes, we guarantee a full refund or store credit if this product arrives damaged beyond usability, or has a genuine performance issue under proper usage and storage, provided you report it immediately with evidence. Claims after the expiration date or due to improper storage after receipt are excluded.
No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.
