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Product details
Catalog number #30002-T
Price for #30002-T
Size / count 1 Kit
The Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells provides green/red fluorescent staining of live and dead cells, respectively. The assay employs two probes that detect intracellular esterase activity in live cells and compromised plasma membrane integrity in dead cells. The esterase substrate calcein AM stains live cells green, while the membrane-impermeable DNA dye Ethidium homodimer III (EthD-III) stains dead cells red. The kit is suitable for detection using fluorescence microscopy, fluorescence microplate reader, or flow cytometry. The assay principles are general and applicable to most eukaryotic cell types, including adherent cells and certain tissues, but not to bacteria or yeast. This fluorescence-based method of assessing cell viability can be used in place of trypan blue exclusion, 51Cr release, and similar methods for determining cell viability and cytotoxicity. EthD-III (#40050 and #40051) is a proprietary DNA stain developed by Biotium to be a superior alternative to the spectrally similar dye Ethidium homodimer I (EthD-I) . EthD-III has higher DNA binding affinity and thus brighter fluorescence (30% brighter than EthD-I) . Spectral Properties Calcein (end product after hydrolysis of calcein AM) : Ex/Em: 494/517 nm (pH 8) Ethidium homodimer III (EthDIII) : Ex/Em: 530/620 nm (with DNA)
The catalog number for Viability/Cytotoxity Assay Kit for Animal Live & Dead Cell (150 assays) is #30002-T.
Viability/Cytotoxity Assay Kit for Animal Live & Dead Cell (150 assays) is available in the following size(s): 1 Kit.
Viability/Cytotoxity Assay Kit for Animal Live & Dead Cell (150 assays) is currently made to order / not held in immediate stock. Contact us for lead time.
Viability/Cytotoxity Assay Kit for Animal Live & Dead Cell (150 assays) is manufactured by Biotium.
Estimated delivery time for Viability/Cytotoxity Assay Kit for Animal Live & Dead Cell (150 assays) is approximately 15 business day(s), depending on your location.
The safety data sheet for Viability/Cytotoxity Assay Kit for Animal Live & Dead Cell (150 assays) is available here: https://cdn.gentaur.com/products/37/699c39d20d8c9d341e16474d/msds/%2330002-t.pdf.
The Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells provides green/red fluorescent staining of live and dead cells, respectively. The assay employs two probes that detect intracellular esterase activity in live cells and compromised plasma membrane integrity in dead cells. The esterase
If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.
The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.
A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.
