EvaGreen® 20X in Water, 1 mL

Catalog number #31000-T

Price for #31000-T

Size / count 1x 1 mL

About this product

EvaGreen® Dye, 20X in Water is a green fluorescent nucleic acid dye designed for real-time PCR (qPCR), DNA melt curve analysis, HRM®, LAMP and digital PCR. The dye is essentially non-fluorescent in its free form and becomes highly fluorescent upon binding to double-stranded DNA (dsDNA), enabling sensitive fluorescence-based DNA detection. The 20X aqueous formulation provides a convenient DNA-binding dye for qPCR workflows and is spectrally similar to SYBR® Green I/FAM, making it compatible with commonly used real-time PCR detection channels.<div> <div>Key Features <ul style=""><li style="">Low PCR Inhibition → EvaGreen® is less inhibitory to PCR than SYBR® Green I, allowing higher dye concentrations and stronger fluorescence signals in suitable qPCR workflows. </li><li style="">High Sensitivity → Its low PCR inhibition enables high dye concentrations for sensitive qPCR detection and robust DNA melt curve analysis. </li><li style="">Nonmutagenic and Noncytotoxic → EvaGreen® has been shown to be non-mutagenic and non-cytotoxic in standard Ames testing and is impermeable to cell membranes. </li><li style="">Compatible with Fast PCR → Minimal PCR interference supports fast PCR protocols where reduced extension times are required. </li><li style="">Multiplex PCR Compatible → EvaGreen® exhibits limited dye redistribution between amplicons at recommended concentrations, supporting multiplex PCR applications and melt-curve-based analysis. </li><li style="">Exceptional Stability → The dye provides high thermal, chemical and photostability, including stability under PCR conditions. </li><li style="">SYBR® Green I-Like Spectral Profile → EvaGreen® can be detected using common SYBR® Green I or FAM channels, facilitating compatibility with major real-time PCR platforms.</li></ul></div></div>

Specifications

Catalog number: #31000-T
Size / count: 1x 1 mL
Dry ice: No
Hazardous: No
Discontinued: No
UNSPSC: 41106307
Origin: Synthetic
Applications: EvaGreen® Dye Applications <ul><li>qPCR / Real-Time PCR → Fluorescent detection of amplified DNA during quantitative PCR. </li><li>DNA Melt Curve Analysis → High-signal fluorescence for post-amplification melt curve analysis. </li><li>High-Resolution Melting (HRM®) → Suitable for DNA melting analysis where high-resolution fluorescence measurements are required. </li><li>Digital PCR → EvaGreen® has been validated for droplet digital PCR applications. </li><li>LAMP → Fluorescent monitoring of loop-mediated isothermal amplification. </li><li>DNA Quantification → Fluorescence-based measurement of double-stranded DNA. </li><li>Gel Electrophoresis → PCR products amplified with EvaGreen® can be visualized directly by gel electrophoresis without an additional nucleic acid staining step.</li></ul>
Stability: 12 months
Storage Conditions: F
Shelf Life: 12 months
FAQ: What is EvaGreen® Dye? → EvaGreen® is a green fluorescent nucleic acid dye that becomes highly fluorescent when bound to double-stranded DNA. It is widely used for qPCR and other DNA amplification and detection applications. What is EvaGreen® 20X in Water used for? → The 20X aqueous formulation is primarily used for qPCR, with additional applications including melt curve analysis, HRM®, LAMP, digital PCR and DNA gel electrophoresis. Is EvaGreen® an alternative to SYBR® Green I? → EvaGreen® can be used as an alternative DNA-binding dye for qPCR applications. It has a similar spectral profile while offering lower PCR inhibition and high stability under PCR conditions. Is EvaGreen® compatible with qPCR instruments? → Yes. EvaGreen® can be detected using common SYBR® Green or FAM channels on compatible real-time PCR instruments. Can EvaGreen® be used for melt curve analysis? → Yes. Its low PCR inhibition and high permitted dye concentration make EvaGreen® particularly suitable for DNA melt curve and HRM® analysis. What size is this EvaGreen® product? → This product is the 1 mL trial-size format, manufacturer catalog number 31000-T, sold by Gentaur under CAT#: 0037-#31000-T

Frequently Asked Questions

What is the catalog number for EvaGreen® 20X in Water, 1 mL?

The catalog number for EvaGreen® 20X in Water, 1 mL is #31000-T.

What sizes are available for EvaGreen® 20X in Water, 1 mL?

EvaGreen® 20X in Water, 1 mL is available in the following size(s): 1x 1 mL.

Is EvaGreen® 20X in Water, 1 mL in stock?

EvaGreen® 20X in Water, 1 mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures EvaGreen® 20X in Water, 1 mL?

EvaGreen® 20X in Water, 1 mL is manufactured by Biotium.

What is the estimated delivery time for EvaGreen® 20X in Water, 1 mL?

Estimated delivery time for EvaGreen® 20X in Water, 1 mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for EvaGreen® 20X in Water, 1 mL?

The safety data sheet for EvaGreen® 20X in Water, 1 mL is available here: https://cdn.gentaur.com/products/37/699c39d20d8c9d341e1647fc/msds/%2331000-t.pdf.

What is EvaGreen® 20X in Water, 1 mL used for?

Features: Features:Low PCR inhibitionHighly SensitiveNonmutagenic and noncytotoxicCompatible with fast PCR protocolsCompatible with multiplex PCRUnsurpassed thermal stability, chemical stability and photostabilitySpectrally similar to SYBR® Green I

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.