EvaGreen®, 2000X in DMSO

Catalog number #31019

Price for #31019

Size / count 1x 50 µL

About this product

EvaGreen&reg; Dye, 2000X in DMSO is offered for customers with specialized applications requiring a concentrated dye solution.&nbsp; &nbsp; Note: for most qPCR applications, we recommend our ready-to-use Forget-Me-Not&trade; EvaGreen&reg; qPCR Master Mixes or EvaGreen&reg; Dye, 20X in water.&nbsp; &nbsp; EvaGreen&reg; dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR&reg; Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen&reg; dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen&reg; dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR&reg; Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer.&nbsp; &nbsp; The unique properties of EvaGreen&reg; dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR&reg; Green I, EvaGreen&reg; dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen&reg; dye can be used at a much higher dye concentration than SYBR&reg; Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.&nbsp; &nbsp; EvaGreen&reg; dye and applications are covered under granted and pending US and international patents. <div> </div><div>Key Features <ul><li>2000X Concentrated Formulation → Highly concentrated EvaGreen® dye in DMSO for specialized applications requiring flexible dye preparation.</li><li>High Fluorescence Upon DNA Binding → Essentially non-fluorescent in solution but becomes highly fluorescent when bound to double-stranded DNA.</li><li>Low PCR Inhibition → EvaGreen® is generally less inhibitory to PCR than SYBR® Green I, allowing higher dye concentrations in suitable amplification workflows.</li><li>High-Sensitivity DNA Detection → Supports strong fluorescence signals for real-time PCR, melt curve analysis and digital PCR applications.</li><li>FAM / SYBR® Green I-Compatible Spectrum → DNA-bound EvaGreen® has excitation and emission spectra close to fluorescein (FAM) and SYBR® Green I, supporting compatibility with instruments using appropriate detection channels.</li><li>Excellent Stability → EvaGreen® exhibits high thermal and hydrolytic stability, supporting routine laboratory handling.</li><li>Suitable for Specialized Applications → Designed for workflows including qPCR, DNA melt curve analysis, tHDA and capillary gel electrophoresis.</li><li>Nonmutagenic and Noncytotoxic → EvaGreen® has been reported as nonmutagenic and noncytotoxic based on manufacturer-reported testing.</li></ul></div>

Specifications

Catalog number: #31019
Size / count: 1x 50 µL
Dry ice: No
Hazardous: No
Discontinued: No
UNSPSC: 41106307
Origin: Synthetic
Applications: EvaGreen® Dye 2000X Applications <ul><li>Real-Time PCR (qPCR) → Fluorescent monitoring and quantification of DNA amplification.</li><li>DNA Melt Curve Analysis → Analysis of DNA melting behavior following amplification.</li><li>Droplet Digital PCR (ddPCR) → High-resolution fluorescence-based detection of DNA in partitioned PCR reactions.</li><li>Thermophilic Helicase-Dependent Amplification (tHDA) → Real-time fluorescence monitoring of DNA amplification using helicase-dependent amplification.</li><li>Capillary Gel Electrophoresis → Fluorescent nucleic acid detection in compatible capillary electrophoresis workflows.</li></ul>
Stability: 12 months
Storage Conditions: R
FAQ: What is EvaGreen® Dye 2000X in DMSO? → EvaGreen® Dye 2000X in DMSO is a highly concentrated fluorescent DNA-binding dye formulated in DMSO for specialized nucleic acid detection and amplification applications. What is EvaGreen® 2000X used for? → It can be used for qPCR, DNA melt curve analysis, droplet digital PCR, thermophilic helicase-dependent amplification and capillary gel electrophoresis. Why is EvaGreen® supplied at 2000X? → The highly concentrated formulation provides flexibility for applications requiring a concentrated EvaGreen® stock solution. It is intended primarily for specialized applications rather than convenience-oriented routine qPCR. Is EvaGreen® an alternative to SYBR® Green I? → Yes. EvaGreen® can serve as an alternative DNA-binding fluorescent dye for qPCR and related applications. It has a similar spectral profile to SYBR® Green I while offering lower PCR inhibition in appropriate workflows. What is the difference between EvaGreen® 2000X in DMSO and EvaGreen® 20X in water? → The main difference is the formulation and concentration. EvaGreen® 2000X is supplied as a highly concentrated DMSO stock for specialized applications, while EvaGreen® 20X in Water provides a lower-concentration aqueous formulation intended for convenient use in common qPCR workflows. What is the size of this EvaGreen® product? → This product is supplied as 1 × 50 µL under Gentaur CAT: 0037-31019.
Classification: Dimethylsulfoxide >99%, CAS 67-68-5

Frequently Asked Questions

What is the catalog number for EvaGreen®, 2000X in DMSO?

The catalog number for EvaGreen®, 2000X in DMSO is #31019.

What sizes are available for EvaGreen®, 2000X in DMSO?

EvaGreen®, 2000X in DMSO is available in the following size(s): 1x 50 µL.

Is EvaGreen®, 2000X in DMSO in stock?

EvaGreen®, 2000X in DMSO is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures EvaGreen®, 2000X in DMSO?

EvaGreen®, 2000X in DMSO is manufactured by Biotium.

What is the estimated delivery time for EvaGreen®, 2000X in DMSO?

Estimated delivery time for EvaGreen®, 2000X in DMSO is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for EvaGreen®, 2000X in DMSO?

The safety data sheet for EvaGreen®, 2000X in DMSO is available here: https://cdn.gentaur.com/products/37/699c39d20d8c9d341e164818/msds/%2331019.pdf.

What is EvaGreen®, 2000X in DMSO used for?

EvaGreen® Dye, 2000X in DMSO is offered for customers with specialized applications requiring a concentrated dye solution.    Note: for most qPCR applications, we recommend our ready-to-use Forget-Me-Not™ EvaGreen® qPCR Master Mixes or EvaGreen® Dye, 20X in water.  &

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.