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Product details

EvaGreen® Plus, 20X in Water, 1 mL

Catalog number #31077-T

Price for #31077-T

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Size / count 1x 1 mL

About this product

<font size="3">EvaGreen® Plus Dye, 20X in Water is a high-sensitivity fluorescent nucleic acid dye designed for real-time PCR (qPCR), high-resolution melt (HRM®), digital PCR and isothermal amplification. As an improved version of the original EvaGreen® Dye, EvaGreen® Plus provides a higher signal-to-noise ratio, brighter fluorescence and lower background, supporting sensitive DNA detection and amplification analysis. The 20X aqueous formulation is supplied as a convenient concentrated stock for compatible DNA amplification workflows. The manufacturer specifies the 1 mL trial format as catalog : <b>0037-#31077-T. </b></font><div><font size="3"><br></font></div><div><font size="5" color="#103094"><b style="">Key Features</b></font></div><div><font size="5" color="#103094"><b style=""> </b></font><ul style=""><li><font size="3"><font color="#0015ab"><b>Higher Signal-to-Noise →</b></font> EvaGreen® Plus provides an improved signal-to-noise ratio compared with the original EvaGreen® Dye, with higher fluorescence signal and lower background. </font></li><li style="font-size: medium;"><font size="3"><font color="#0015ab"><b>High-Sensitivity DNA Detection →</b></font> The improved fluorescence performance supports sensitive detection of amplified DNA in real-time PCR and other nucleic acid amplification applications. </font></li><li style="font-size: medium;"><font size="3"><font color="#0015ab"><b>qPCR Compatible →</b></font> Designed for quantitative real-time PCR workflows using DNA-binding dye detection. </font></li><li style="font-size: medium;"><font size="3"><font color="#0015ab"><b>HRM® Compatible →</b></font> Suitable for high-resolution melt analysis, where strong fluorescence and low background are important for resolving DNA melting behavior. </font></li><li style="font-size: medium;"><font size="3"><b><font color="#0015ab">Digital PCR Compatible → </font></b>The higher signal-to-noise performance provides advantages for digital PCR applications. </font></li><li style="font-size: medium;"><b><font color="#0015ab">LAMP Compatible → </font></b>Suitable for fluorescence-based monitoring of loop-mediated isothermal amplification.</li><li style="font-size: medium;"><font size="3"><b><font color="#0015ab">Improved Instrument Compatibility →</font></b> EvaGreen® Plus has a better match for common qPCR instrument excitation sources.</font></li><li style="font-size: medium;"><font size="3"><b><font color="#0015ab">Excellent Stability →</font></b> The dye is designed to maintain stability during storage and PCR conditions.</font></li></ul><font size="3"><b></b></font></div>

Specifications

Catalog number
#31077-T
Size / count
1x 1 mL
Dry ice
No
Hazardous
No
Discontinued
No
UNSPSC
41106307
Origin
Synthetic
Applications
<font size="5" color="#133094"><b>EvaGreen® Plus Dye Applications </b></font><font size="3"> </font><ul><li><font size="3"><b><font color="#0015ab">Real-Time PCR (qPCR) →</font></b> Fluorescent monitoring and quantification of DNA amplification. </font></li><li><font size="3"><b><font color="#0015ab">High-Resolution Melt Analysis (HRM®) →</font></b> Detection and analysis of DNA melting profiles following amplification. </font></li><li><font size="3"><b><font color="#0015ab">Digital PCR →</font></b> Fluorescent DNA detection in partition-based digital PCR workflows. </font></li><li><font size="3"><b><font color="#0015ab">LAMP →</font></b> Fluorescence-based monitoring of isothermal DNA amplification. </font></li><li><font size="3"><b><font color="#0015ab">DNA Amplification Research → </font></b>Suitable for molecular biology workflows requiring sensitive fluorescent detection of amplified DNA. </font></li></ul><div><font size="3"><br></font></div><font size="3">The manufacturer specifically identifies qPCR, HRM®, digital PCR and LAMP among the principal applications of EvaGreen® Plus.</font>
Stability
12 months
Features & Benefits
Brighter fluorescence and lower background compared to original EvaGreen
Storage Conditions
F
FAQ
<font size="3"><font color="#0015ab"><b>What is EvaGreen® Plus Dye? </b></font><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0015ab" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">→ </font></span>EvaGreen® Plus is an improved fluorescent DNA-binding dye designed for sensitive DNA amplification and detection applications including qPCR, HRM®, digital PCR and LAMP. <font color="#0015ab"><b>What is EvaGreen® Plus 20X in Water used for? </b></font><span style="color: rgb(0, 21, 171); font-weight: 700;">→ </span>The 20X aqueous formulation is primarily used as a concentrated fluorescent dye for real-time PCR and DNA amplification applications, including HRM®, digital PCR and LAMP. <font color="#0015ab"><b>What is the difference between EvaGreen® and EvaGreen® Plus? </b></font><span style="color: rgb(0, 21, 171); font-weight: 700;">→ </span>EvaGreen® Plus is an improved version of the original EvaGreen® Dye, offering higher signal-to-noise, lower background and higher fluorescence signal. <font color="#0015ab"><b>Can EvaGreen® Plus be used for HRM? </b></font><span style="color: rgb(0, 21, 171); font-weight: 700;">→ </span>Yes. EvaGreen® Plus is compatible with high-resolution melt (HRM®) analysis. <font color="#0015ab"><b>Can EvaGreen® Plus be used for digital PCR? </b></font><span style="color: rgb(0, 21, 171); font-weight: 700;">→ </span>Yes. The improved signal-to-noise ratio provides advantages for digital PCR applications. <b><font color="#0015ab">What is the concentration of EvaGreen® Plus? </font></b><span style="color: rgb(0, 21, 171); font-weight: 700;">→ </span>EvaGreen® Plus is supplied at 20X concentration.</font>

Frequently Asked Questions

What is the catalog number for EvaGreen® Plus, 20X in Water, 1 mL?

The catalog number for EvaGreen® Plus, 20X in Water, 1 mL is #31077-T.

What sizes are available for EvaGreen® Plus, 20X in Water, 1 mL?

EvaGreen® Plus, 20X in Water, 1 mL is available in the following size(s): 1x 1 mL.

Is EvaGreen® Plus, 20X in Water, 1 mL in stock?

EvaGreen® Plus, 20X in Water, 1 mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures EvaGreen® Plus, 20X in Water, 1 mL?

EvaGreen® Plus, 20X in Water, 1 mL is manufactured by Biotium.

What is the estimated delivery time for EvaGreen® Plus, 20X in Water, 1 mL?

Estimated delivery time for EvaGreen® Plus, 20X in Water, 1 mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for EvaGreen® Plus, 20X in Water, 1 mL?

The safety data sheet for EvaGreen® Plus, 20X in Water, 1 mL is available here: https://cdn.gentaur.com/products/37/699c39d20d8c9d341e164860/msds/%2331077-t.pdf.

What is EvaGreen® Plus, 20X in Water, 1 mL used for?

EvaGreen® Plus Dye is an improved alternative to Biotium’s original EvaGreen® Dye. EvaGreen® Plus Dye has an improved signal-to-noise ratio and better instrument compatibility that is more advantageous for a variety of DNA detection applications, including qPCR, digital PCR, and LAMP.

Do you provide a Certificate of Analysis (CoA) for this product?

A Certificate of Analysis is available for most products upon request. Contact our support team with the lot number to obtain the relevant CoA.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

Is this product covered by a money-back guarantee?

Yes, we guarantee a full refund or store credit if this product arrives damaged beyond usability, or has a genuine performance issue under proper usage and storage, provided you report it immediately with evidence. Claims after the expiration date or due to improper storage after receipt are excluded.

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