Pan-Lactyl-lysine Recombinant Rabbit Monoclonal Antibody [PSH03-73]

About this product

Post-translational modifications (PTMs) represent a crucial means of regulating diverse biological processes and cellular physiology by influencing protein structure and function. Histone lysine lactylation (Kla) is a newly discovered histone modification, which regulates gene expression in macrophages. In M1 macrophages, lactate is derived from incompletely oxidized glucose and then generate lactyl-CoA, which is transferred to lysine tails of histone proteins via the acetyl transferase p300. This modification was high in gene promoter regions that lack acetylation and associated with activation of genes expression. As a large number of studies have demonstrated that lysine acylation possessed a diverse range of substrate proteins, however, no systematic analysis has been reported for lysine lactylation.

Specifications

Catalog number

HA722037

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Lactyllysine antibody

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Universal

Immunogen

Synthetic Lactyl lysine-containing peptide.

Isotype

IgG

Clone

PSH03-73

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell, FC, Dot Blot

Positive Control

HeLa treated with 100mM Lactate sodium for 24 hours cell lysate, human breast cancer tissue, human colon cancer tissue, mouse small intestine tissue, rat small intestine tissue, HeLa cells treated with 100mM Lactate sodium for 24 hours.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IHC-P: 1:500-1:1,000 ;IF-Cell: 1:1,000 ;FC: 1:1,000 ;Dot Blot: 1:2,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes