AF/LE Purified Anti-Mouse CD150 Antibody[TC15-12F12.2]

Specifications

Catalog number

E-AB-F11770-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Abbreviation

CD150

Gene ID

27218

UniProt

Q9QUM4

Host

Rat

Reactivity

Mouse

Immunogen

Recombinant Mouse CD150 protein

Target

SLAM family member; SLAMF; CD 150; CD150; CD150 antigen; Signaling lymphocytic activation molecule; SLAM family member 1; Slam; Slamf1; IPO-3

Clonality

Monoclonal

Clone

TC15-12F12.2

Conjugation

None (AF/LE)

Applications

FCM

Purification

>98%, Protein A/G purified

Concentration

≥ 1 mg/mL

Dilution

66

Buffer

Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Isotype

Rat IgG2a, λ