Recombinant Human E-Selectin Protein (His Tag)

Specifications

Catalog number

PDMH100429-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed on the surfaces of stimulated vascular endothelial cells and is sometimes involved in cancer cell metastasis. E-selectin exhibits a complex mosaic structure consisting of a large extracellular region comprised of a lectin domain, an EGF-like domain, and a short consensus repeat (SCR) domain, followed by a transmembrane region and a relatively short (32 aa) cytoplasmic tail. As a member of the LEC-CAM or selectin family, E-selectin recognises and binds to sialylated carbohydrates including members of the Lewis X and Lewis A families found on monocytes, granulocytes, and T-lymphocytes. E-selectin supports rolling and stable arrest of leukocytes on activated vascular endothelium, and furthermore, it was indicated that it can also transduce an activating stimulus via the MAPK cascade into the endothelial cell during leukocyte adhesion. E-selectin regulates adhesive interactions between certain blood cells and endothelium. The soluble form of E selectin (sE-selectin) is a marker of endothelial activation, and has a potential role in the pathogenesis of cardiovascular disease as raised levels have been found in hypertension, diabetes and hyperlipidemia, although its association in established atherosclerosis disease and its value as a prognostic factor is more controversial. soluble E-selectin is inversely associated with the muscular component of the left ventricle, thereby suggesting that the lack of such a reparative factor may be associated with cardiac remodeling in end-stage renal disease (ESRD) patients. Besides, this adhesion molecule appears to be involved in the pathogenesis of atherosclerosis.

Abbreviation

E-Selectin

Synonyms

LYAM; CD62E and ELAM; Endothelial adhesion molecule; Endothelial leukocyte adhesion molecule; Leukocyte-endothelial cell adhesion molecule; LECAM; SELE; CD62E; ELAM; ELAM1; ESEL; LECAM2; CD62E and ELAM1; E selectin; ELAM-1; Endothelial adhesion molecule 1; E-selectin (SELE) ; Leukocyte-endothelial cell adhesion molecule 2; Selectin E; E-selectin; LYAM2; CD26E; Endothelial leukocyte adhesion molecule 1; CD62 antigen-like family member E; Sialyl Lewis X; Ceramide-dodecasaccharide; ELAM-1; LECAM-2

UniProt

P16581

Accession Number

P16581

Expression System

Mammalian

Tag

C-His

Sequence

Met1-Pro556

Field of Research

Cancer; Cardiovascular; Cell Biology; Immunology; Signal Transduction

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

61 kDa

Observed Molecular Weight

80-100 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human