Recombinant Human BAX Protein (Trx Tag)

Specifications

Catalog number

PDEH100644-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

BCL2 associated X (BAX) is traditionally thoμght to be regulated by anti-apoptotic BCL-2 family members. BCL-2-associated X protein (BAX) is a critical apoptotic regulator that can be transformed from a cytosolic monomer into a lethal mitochondrial oligomer, The pro-apoptotic BCL-2 protein BAX commits human cells to apoptosis by permeabilizing the outer mitochondrial membrane. BAX activation has been sμggested to require the separation of helix alpha5 from alpha6 - the 'latch' from the 'core' domain - among other conformational changes. BCL-2-associated X (BAX) protein acts as a gatekeeper in regulating mitochondria-dependent apoptosis. Under cellular stress, BAX becomes activated and transforms into a lethal oligomer that causes mitochondrial outer membrane permeabilization (MOMP) .

Abbreviation

BAX

Synonyms

BCL2L4bcl2-L; Bcl-2-like protein; BCL2L; Bcl2-L; BAX; BCL2L4; apoptosis regulator BAX; Bcl2-L-4; BCL2L4bcl2-L-4; BCL2-associated X protein; Bcl-2-like protein 4; BAXA; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; Bax-protein; BCL2 associated X protein; BCL2 associated X protein omega; BCL2 associated X protein transcript variant delta2; Bcl-2-like protein 4 (Bcl2-L-4) ; membrane isoform alpha

UniProt

Q07812

Accession Number

Q07812

Expression System

E.coli

Tag

N-Trx

Sequence

Met1-Trp158

Field of Research

Cell Biology; Metabolism; Cancer; Kits; Lysates; Other

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

37.4 kDa

Observed Molecular Weight

35 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human