Acetylated-Lysine Recombinant Rabbit Monoclonal Antibody [PSH09-15]

About this product

Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) . Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis. Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of post-translational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport.

Specifications

Catalog number

HA723073

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Host

Rabbit

Species Reactivity

Universal

Immunogen

Synthetic Acetylated lysine-containing peptide.

Isotype

IgG

Clone

PSH09-15

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Tissue, ChIP

Positive Control

HeLa cell lysate, HeLa treated with 1μM TSA for 18 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 400nM TSA for 18 hours cell lysate, C6 cell lysate, C6 treated with 1μM TSA for 18 hours cell lysate, human breast cancer tissue, human colon cancer tissue, mouse liver tissue, rat liver tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IHC-P: 1:20,000-1:100,000 ;IF-Tissue:1:5,000-1:20,000 ;ChIP: Use 5 μg for 25 μg of chromatin.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes