CD14 Recombinant Rabbit Monoclonal Antibody [PD01-63]

About this product

CD14 is a 55-kDa protein found as a glycosylphosphatidylinositol (GPI)- anchored protein on the surface of monocytes, macrophages, and polymorphonuclear leukocytes, and as a soluble protein in the blood. Its main function is to serve as a receptor for lipopolysaccharide (LPS). CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the "adapter molecule," lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14. Besides its role in endotoxin signaling, it has been proposed that CD14 is involved in the transportation of other lipids, cell-cell interactions during different immune responses, and recognition of apoptotic cells. CD14 is highly expressed on the surface of monocytes/macrophages and strongly up-regulated during the differentiation of monocytic precursor cells into mature monocytes. Therefore, CD14 has been commonly used as a differentiation marker for monocytes/macrophages. An antibody to CD14 also labels Langerhans' cells and dendritic cells.

Specifications

Catalog number

HA721321

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CD 14 antibody CD_antigen=CD14 antibody CD14 antibody CD14 antigen antibody CD14 molecule antibody CD14_HUMAN antibody LPS-R antibody Mo2 antibody Monocyte differentiation antigen CD14 antibody Monocyte differentiation antigen CD14 urinary form antibody Monocyte differentiation antigen CD14, membrane-bound form antibody Myeloid cell specific leucine rich glycoprotein antibody Myeloid cell-specific leucine-rich glycoprotein antibody

Abbreviation

CD14

Swiss Prot

P08571 Human, P10810 Mouse

Cellular Locus

Cell membrane, Secreted, Golgi apparatus, Membrane raft.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within human CD14 aa 300-375.

Isotype

IgG

Clone

PD01-63

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

NIH/3T3 cell lysate, SiHa cell lysate, human colon carcinoma tissue, human liver tissue, human tonsil tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:200-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 40 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes