SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag

Catalog number NUN-C52Hs-1mg

Discontinued

Price for NUN-C52Hs-1mg

Size / count 1 mg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag (NUN-C52Hs) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (D63G, R203M, D377Y )). The mutations (D63G, R203M, D377Y) were identified in the SARS-CoV-2 Delta lineages (Pango lineage: B.1.617.2; GISAID clade: 21A, 21I, 21J; Nextstrain clade: G/478K.V1).

Specifications

Catalog number: NUN-C52Hs-1mg
Size / count: 1 mg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.3 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Met 1 - Ala 419
Host: HEK 293
Target: Nucleocapsid protein
Conjugation: Unconjugated
Tag: C-6×His
Source: SARS-CoV-2
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag (Cat. No. NUN-C52Hs) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 with a linear range of 0.1-1 ng/mL (QC tested).
Format: Powder
Buffer: PBS,pH7.3 with 0.2 M Arginine
Additives: Trehalose
Molecular Weight: 47.3 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag?

The catalog number for SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag is NUN-C52Hs-1mg.

Is SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag in stock?

SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag?

SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag?

Estimated delivery time for SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y), His Tag used for?

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.