Lipolysis Colorimetric Assay Kit

Specifications

Catalog number

E-BC-K798-M-01

Size / count

48 T (32 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Type

Colorimetric method

Field of Research

Glycolysis and Lipid Metabolism; Metabolic Diseases

Assay Principle

Lipolysis is the process by which the body converts fat reserves into energy, the fat stored in fat cells is gradually hydrolyzed under the catalysis of lipase, releasing free fatty acid (FFA) and glycerin (Gly) . The glycerol produced by lipolysis cannot be used by the cells themselves, and needs to be transported to the liver for further conversion into energy. The content of glycerol can reflect the level of lipolysis in the cell sample. The detection principle of this kit is to make the glycerol released by cells produce hydrogen peroxide under the catalysis of lipase, and hydrogen peroxide reacts with enzymes and chemical substances to produce specific products, which have maximum absorption at 555 nm, and OD value is proportional to the concentration within a certain range.

Assay Performance Time

30 min

Sample Type

Cell

Detection Range

0.005-0.543 mmol/L

Sensitivity

0.005 mmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

1.6-2.5%

Intra CV

1.3-1.4%

Detection Instrument

Microplate reader