Pericentrin Recombinant Rabbit Monoclonal Antibody [PSH0-66]

About this product

Pericentrin (kendrin), also known as PCNT and pericentrin-B (PCNTB), is a protein which in humans is encoded by the PCNT gene on chromosome 21. This protein localizes to the centrosome and recruits proteins to the pericentriolar matrix (PCM) to ensure proper centrosome and mitotic spindle formation, and thus, uninterrupted cell cycle progression. This gene is implicated in many diseases and disorders, including congenital disorders such as microcephalic osteodysplastic primordial dwarfism type II (MOPDII) and Seckel syndrome. The protein encoded by this gene is expressed in the cytoplasm and centrosome throughout the cell cycle, and to a lesser extent, in the nucleus. It is an integral component of the PCM, which is a centrosome scaffold that anchors microtubule nucleating complexes and other centrosomal proteins. In one model, PCNT complexes with CEP215 and is phosphorylated by PLK1, leading to PCM component recruitment and organization, centrosome maturation, and spindle formation. The protein controls the nucleation of microtubules by interacting with the microtubule nucleation component γ-tubulin, thus anchoring the γ-tubulin ring complex to the centrosome, which is essential for bipolar spindle formation and chromosome assembly in early mitosis. This ensures normal function and organization of the centrosomes, mitotic spindles, and cytoskeleton, and by extension, regulation over cell cycle progression and checkpoints. Downregulation of PCNT disrupted mitotic checkpoints and arrested the cell at the G2/M checkpoint, leading to cell death. Moreover, microtubule functioning was also disrupted, resulting in mono- or multipolar spindles, chromosomal misalignment, premature sister chromatid separation, and aneuploidy. PCNT is highly abundant in skeletal muscle, indicating that it may be involved in muscle insulin action. PCNT is also involved in neuronal development through its interaction with DISC1 to regulate microtubule organization.

Specifications

Catalog number

HA721454

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Centrosome Marker antibody Ken antibody Kendrin antibody KIAA0402 antibody MOPD2 antibody PCN antibody PCNT 2 antibody PCNT antibody PCNT B antibody PCNT_HUMAN antibody PCNT1 antibody PCNT2 antibody PCNTB antibody PCTN2 antibody Pericentrin 1 antibody Pericentrin 2 antibody Pericentrin 380 antibody Pericentrin antibody Pericentrin B antibody Pericentrin-B antibody SCKL4 antibody

Abbreviation

PCNT, KIAA0402, PCNT2

Swiss Prot

O95613 Human

Cellular Locus

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within human aa 201-600 / 3,336.

Isotype

IgG

Clone

PSH0-66

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB

Positive Control

HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, K-562 cell lysate.

Concentration

1 mg/mL

Dilution

WB: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 378 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes