Nesfatin-1/Nucleobindin-2 Polyclonal Antibody

Specifications

Catalog number

AN005880L-01

Size / count

25 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Nesfatin-1 (NEFA Encoded Satiety and Fat-influencing protein 1) is a secreted, 10 kDa peptide derived from the translation product of the NUCB2 gene. Nesfatin-1 is associated with neurons involved in feeding (ARH and PVH) and fluid intake (SON and PVH), in beta cells of the pancreas, and in adipocytes. Its presence peripherally has an anorexigenic effect. Mature human Nesfatin-1 is 82 amino acids (aa) in length. It is the secreted N-terminus of a larger, intracellular 420 aa precursor termed NEFA/Nucleobindin-2/NUCB2. NUCB2 contains a signal sequence (aa 1- 24), Nesfatin-1 (aa 25-106), a DNA-binding site (aa 171-223), and two EF-hand regions (aa 241-276 and 293-328) . Intracellular uncleaved NUCB2 is 45-50 kDa in size. There is a potential for multiple cleavages, generating Nesfatin-2 (aa 109-187) and Nesfatin-3 (aa 190-420) . Human Nesfatin-1 shares 85% aa identity with mouse Nesfatin-1.

Abbreviation

Nesfatin-1/Nucleobindin-2

UniProt

P80303

Host

Rabbit

Reactivity

Human; Mouse; Rat

Immunogen

Recombinant Human Nesfatin-1/Nucleobindin-2 protein expressed by E.coli

Target

DNA-binding protein NEFA; NEFA; NEFAGastric cancer antigen Zg4; Nesfatin1; Nesfatin-1; NUCB2; nucleobindin 2; Nucleobindin-2; nucleobinding 2

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

IHC

Field of Research

Signal Transduction; Epigenetics and Nuclear Signaling

Purification

Antigen Affinity Purification

Concentration

1 mg/mL

Dilution

IHC 1:500-1:1000

Buffer

PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

Isotype

IgG