PABP Recombinant Rabbit Monoclonal Antibody [PSH05-51]

Catalog number HA722289

Price for HA722289

Size / count 100 µL

About this product

The poly (A)-binding protein (PAB or PABP), which is found complexed to the 3' poly (A) tail of eukaryotic mRNA, is required for poly (A) lengthening and the termination of translation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family of other poly (A)-binding proteins. PABPC1 is usually diffused within the cytoplasm and concentrated at sites of high mRNA concentration such as stress granules, processing bodies, and locations of high translational activity. PABPC1 is also associated with nonsense-mediated mRNA decay (NMD). PABPC1 binds to the poly (A) tail and interact with eIF4G, which stabilizes the circularization of mRNAs. This structure is required for the prevention of mRNA degradation via NMD. In the nucleus PABP1 binds to the poly (A) tails of pre-mRNAs to facilitate stability, export, transport, and degradation. PABP1 binding is also required for nuclear-mediated degradation. PABPC1 contains four RNA-recognition motifs (RRMs). The first two, RRM1 and RRM2, bind both α-importin and the poly (A) tail of processed mRNA. This feature prevents mRNA from going back into the nucleus.

Specifications

Catalog number: HA722289
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: PAB 1 antibody PAB1 antibody PABP 1 antibody PABP-1 antibody PABP1 antibody PABP1_HUMAN antibody PABPC 1 antibody Pabpc1 antibody PABPC2 antibody PABPL1 antibody Poly A binding protein 1 antibody Poly A binding protein cytoplasmic 1 antibody poly (A) binding protein, cytoplasmic 1 antibody poly (A) binding protein, cytoplasmic 2 antibody Poly (A)-binding protein 1 antibody Polyadenylate binding protein 1 antibody Polyadenylate-binding protein 1 antibody
Abbreviation: PABPC1, PAB1, PABP, PABP1, PABPC2
Swiss Prot: P11940 Human, P29341 Mouse
Cellular Locus: Cytoplasm, Stress granule, Nucleus, Cell projection, lamellipodium.
Host: Rabbit
Species Reactivity: Human, Mouse, Monkey
Immunogen: Recombinant protein within Human PABP protein aa 450-550.
Isotype: IgG
Clone: PSH05-51
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell, IHC-P, FC, IF-Tissue
Positive Control: TF-1 cell lysate, HeLa cell lysate, A549 cell lysate, MCF7 cell lysate, HepG2 cell lysate, HCT 116 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, COS-1 cell lysate, Vero cell lysate, mouse brain tissue lysate, HeLa, NIH/3T3, human colon tissue, human testis tissue, mouse testis tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:1,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000 ;FC: 1:1,000 ;IF-Tissue: 1:200
Purity: Protein A affinity purified.
Form: Liquid
Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 71 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for PABP Recombinant Rabbit Monoclonal Antibody [PSH05-51]?

The catalog number for PABP Recombinant Rabbit Monoclonal Antibody [PSH05-51] is HA722289.

What sizes are available for PABP Recombinant Rabbit Monoclonal Antibody [PSH05-51]?

PABP Recombinant Rabbit Monoclonal Antibody [PSH05-51] is available in the following size(s): 100 µL.

Is PABP Recombinant Rabbit Monoclonal Antibody [PSH05-51] in stock?

PABP Recombinant Rabbit Monoclonal Antibody [PSH05-51] is currently made to order / not held in immediate stock. Contact us for lead time.

What is PABP Recombinant Rabbit Monoclonal Antibody [PSH05-51] used for?

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.