NifH | Positive control/quantitation standard

Catalog number AS01 021S

Price for AS01 021S

Size / count 100 µL

Specifications

Catalog number: AS01 021S
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
Background: Nitrogenase is involved in biological fixation of nitrogen to assimilable ammonia.This product is a recombinant protein standard, source: Nostoc/Anabaena 7120.
CAS Number: 9007-83-4
HS Code: 38221900
Not reactive in: No confirmed exceptions from predicted reactivity are currently known.
Applications: Western blot (WB)
Field of Research: Positive controls/Quantitation standards
Dilution: Standard curve: 3 loads are recommended (0.5, 2 and 4 μl) .For most applications a sample load of 0.2 μg of chlorophyll will give a NifH signal in this range.Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems. This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Format: Lyophilized
Reconstitution: For reconstitution add 95 µl of sterile water. Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
Molecular Weight: 34 kDa (larger than a native protein due to the addition of His-tag)
Additionnal Information: The NifH protein standard can be used in combination with global anti-NifH antibodies to quantitate NifH protein from a wide range of cyanobacterial species. Global antibodies are raised against highly conserved 15 amino acid sequence found in NifH proteins.Quantitative western blot: detailed method description, video tutorial
References & Citations: Levitan et a. (2010) . Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO concentrations. Environ. Microbiol (Epub ahead of print)
Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Frequently Asked Questions

What is the catalog number for NifH | Positive control/quantitation standard?

The catalog number for NifH | Positive control/quantitation standard is AS01 021S.

What sizes are available for NifH | Positive control/quantitation standard?

NifH | Positive control/quantitation standard is available in the following size(s): 100 µL.

Is NifH | Positive control/quantitation standard in stock?

NifH | Positive control/quantitation standard is currently made to order / not held in immediate stock. Contact us for lead time.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.