VDAC1 Monoclonal Antibody

Specifications

Catalog number

AN005380L-01

Size / count

25 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol. In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. May mediate ATP export from cells. Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix. Mediates cytochrome c efflux. Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.

Abbreviation

VDAC1

UniProt

P21796

Host

Mouse

Reactivity

Human; Mouse; Rat

Immunogen

Synthetic peptide corresponding to Human VDAC1 protein

Target

VDAC1; Non-selective voltage-gated ion channel VDAC1; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31HL; Porin 31HM; Voltage-dependent anion-selective channel protein 1 (VDAC-1; hVDAC1) ; VDAC

Clonality

Monoclonal

Clone

Da-2G11

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Tags & Cell Markers; Signal Transduction; Isotype; Loading Controls; Metabolism

Purification

Protein A/G Purification

Concentration

1 mg/mL

Dilution

WB 1:1000-1:2000; IHC 1:200-1:400

Buffer

PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

Calculated Molecular Weight

31 kDa

Observed Molecular Weight

33 kDa

Isotype

IgG1