Phospho-Tuberin (T1462) Recombinant Rabbit Monoclonal Antibody [JE43-81]

Catalog number HA721792

Price for HA721792

Size / count 100 µL

About this product

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development. Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors. Tuberin is directly phosphorylated at Thr1462 by Akt/PKB. Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity. In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation. Tuberin is phosphorylated on Ser939 and Thr1462 in response to PI3K activation and that the human TSC complex is a direct biochemical target of the PI3K/Akt pathway. This data complements Drosophila genetics studies suggesting the possible involvement of the tuberin-hamartin complex in the PI3K/Akt mediated insulin pathway.

Specifications

Catalog number: HA721792
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: FLJ43106 antibody LAM antibody OTTHUMP00000158940 antibody OTTHUMP00000198394 antibody OTTHUMP00000198395 antibody PPP1R160 antibody Protein phosphatase 1, regulatory subunit 160 antibody TSC complex subunit 2 antibody tsc2 antibody TSC2_HUMAN antibody TSC4 antibody TSC4 gene, formerly antibody TSC4, formerly antibody Tuberin antibody Tuberous sclerosis 2 antibody Tuberous sclerosis 2 protein antibody Tuberous sclerosis 2 protein homolog antibody
Abbreviation: TSC2, TSC4
Swiss Prot: P49815 Human, Q61037 Mouse, P49816 Rat
Cellular Locus: Cytoplasm, Membrane.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic phosphopeptide corresponding to residues around Thr1462 of human tuberin.
Isotype: IgG
Clone: JE43-81
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB
Positive Control: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate.
Concentration: 1 mg/mL
Dilution: WB: 1:1,000
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 201 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Phospho-Tuberin (T1462) Recombinant Rabbit Monoclonal Antibody [JE43-81]?

The catalog number for Phospho-Tuberin (T1462) Recombinant Rabbit Monoclonal Antibody [JE43-81] is HA721792.

What sizes are available for Phospho-Tuberin (T1462) Recombinant Rabbit Monoclonal Antibody [JE43-81]?

Phospho-Tuberin (T1462) Recombinant Rabbit Monoclonal Antibody [JE43-81] is available in the following size(s): 100 µL.

Is Phospho-Tuberin (T1462) Recombinant Rabbit Monoclonal Antibody [JE43-81] in stock?

Phospho-Tuberin (T1462) Recombinant Rabbit Monoclonal Antibody [JE43-81] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Phospho-Tuberin (T1462) Recombinant Rabbit Monoclonal Antibody [JE43-81] used for?

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.