Elab Fluor® 488 Anti-Mouse CD146 Antibody[ME-9F1]

Specifications

Catalog number

E-AB-F1395L-01

Size / count

50 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. CD146 is strongly expressed by murine vascular endothelial cells. It is expressed on about 30% of neutrophils and 60% of NK cells. Unlike in humans, CD146 is undetectable on monocytes, dendritic cells, T cells, NKT cells, B cells, or smooth muscle cells in mouse. It has been reported that an increase in CD146 expression is associated with NK cell maturation. Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. CD146+ NK cells were found to be less cytotoxic and to produce less IFNγ than CD146- NK cells upon stimulation with target cells or activating antibodies. The role of CD146 on NK cell migration has yet to be investigated. The identification of CD146 ligand (s) will be crucial to address this issue.

Abbreviation

CD146

Gene ID

84004

UniProt

P10810

Host

Rat

Reactivity

Mouse

Target

S-Endo 1 antigen; MUC18; MCAM; Mel-CAM; A32 antigen

Clonality

Monoclonal

Clone

ME-9F1

Conjugation

Elab Fluor®488

Applications

FCM

Concentration

5 μL/Test

Buffer

Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.

Recommended Usage

The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood) .

Isotype

Rat IgG2a, κ