QuicKey Pro Mouse ARG1 (Arginase I) ELISA Kit

Specifications

Catalog number

E-OSEL-M0024-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

ARG1

UniProt

Q61176

Cross Reactivity

This kit recognizes ARG1 in samples.No significant cross-reactivity or interference between ARG1 and analogues was observed.

Target

Liver Arginase

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ARG1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse ARG1 are added to the micro ELISA plate wells. Mouse ARG1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse ARG1.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ARG1. The concentration of Mouse ARG1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

46.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Detection Instrument

Microplate reader

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse ARG1 concentrations in serum, plasma and other biological fluids.