PI3 Kinase p85 alpha Recombinant Rabbit Monoclonal Antibody [JE04-11]

About this product

Phosphatidylinositol 3-kinase regulatory subunit alpha is an enzyme that in humans is encoded by the PIK3R1 gene. Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. The Pik3r1 gene locus encodes the 85 kD regulatory subunit, as well as 55 and 50 kD regulatory subunits. It used to be thought that alternative splicing of this gene resulted in three transcript variants encoding different isoforms. In fact, it has since been shown that the 55 and 50kD subunits have their own promotors within the gene locus Pik3r1. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Suppression specifically of the 85kD subunit in early murine embryoid body development results in a transient cell-cell adhesion deficiency, mediated by transient downregulation of the adhesion molecule integrin-beta1 (ITGB1).

Specifications

Catalog number

HA722522

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

GRB1 antibody p85 alpha antibody p85 antibody P85A_HUMAN antibody Phosphatidylinositol 3 kinase associated p 85 alpha antibody Phosphatidylinositol 3 kinase regulatory 1 antibody Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody Phosphatidylinositol 3-kinase regulatory subunit alpha antibody Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody PI3 kinase p85 antibody PI3 kinase p85 subunit alpha antibody PI3-kinase regulatory subunit alpha antibody PI3-kinase subunit p85-alpha antibody PI3K antibody PI3K p85 antibody PI3K regulatory subunit alpha antibody Pik3r1 antibody PtdIns 3 kinase p85 alpha antibody PtdIns-3-kinase regulatory subunit alpha antibody PtdIns-3-kinase regulatory subunit p85-alpha antibody

Abbreviation

PIK3R1, GRB1

Swiss Prot

P27986 Human, P26450 Mouse, Q63787 Rat

Cellular Locus

Cytoplasm, membrane, nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within

Isotype

IgG

Clone

JE04-11

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB

Positive Control

Jurkat cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate.

Concentration

1 mg/mL

Dilution

WB: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 84 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes