Laminin gamma 1 Recombinant Rabbit Monoclonal Antibody [JE40-75]

About this product

Laminin subunit gamma-1 is a protein that in humans is encoded by the LAMC1 gene. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis.

Specifications

Catalog number

HA721662

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

LAMB2 antibody lamc1 antibody LAMC1_HUMAN antibody Laminin B2 chain antibody Laminin subunit gamma-1 antibody Laminin, gamma 1 (formerly LAMB2) antibody Laminin-1 subunit gamma antibody Laminin-10 subunit gamma antibody Laminin-11 subunit gamma antibody Laminin-2 subunit gamma antibody Laminin-3 subunit gamma antibody Laminin-4 subunit gamma antibody Laminin-6 subunit gamma antibody Laminin-7 subunit gamma antibody Laminin-8 subunit gamma antibody Laminin-9 subunit gamma antibody MGC87297 antibody S-LAM gamma antibody S-laminin subunit gamma antibody

Abbreviation

LAMC1, LAMB2

Swiss Prot

P11047 Human

Cellular Locus

Secreted, extracellular space, extracellular matrix, basement membrane.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human Laminin gamma 1 aa 21-70 / 1,609.

Isotype

IgG

Clone

JE40-75

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB

Positive Control

HeLa cell lysate, A431 cell lysate, OVCAR-3 cell lysate, A549 cell lysate.

Concentration

1 mg/mL

Dilution

WB: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 178 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes