IKK beta Recombinant Rabbit Monoclonal Antibody [JE32-81]

About this product

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or rotection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NAA10, NCOA3, BCL10 and IRS1 . Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus.

Specifications

Catalog number

HA721715

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

I kappa B kinase 2 antibody I kappa B kinase beta antibody I-kappa-B kinase 2 antibody I-kappa-B-kinase beta antibody IkBKB antibody IKK beta antibody IKK-B antibody IKK-beta antibody IKK2 antibody IKKB antibody IKKB_HUMAN antibody IMD15 antibody Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody Inhibitor of nuclear factor kappa-B kinase subunit beta antibody NFKBIKB antibody Nuclear factor NF-kappa-B inhibitor kinase beta antibody

Abbreviation

IKBKB, IKKB

Swiss Prot

O14920 Human

Cellular Locus

Cytoplasm, Nucleus, Membrane raft.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human IKK beta aa 1-50 / 756.

Isotype

IgG

Clone

JE32-81

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, FC

Positive Control

K-562 cell lysate, HL-60 cell lysate, THP-1 cell lysate, Jurkat cell lysate, HeLa cell lysate, THP-1.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;FC: 1:50

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 87 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes