Mini Sample Human LEP (Leptin) ELISA Kit

Specifications

Catalog number

E-MSEL-H0003-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

LEP

UniProt

P41159

Cross Reactivity

This kit recognizes LEP in samples.No significant cross-reactivity or interference between LEP and analogues was observed.

Target

LEPD; OB; OBS; Obesity Homolog

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Stem cells; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LEP . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LEP , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of LEP . You can calculate the concentration of LEP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

39.06-2500 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

14.09pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human LEP concentrations in serum, plasma and other biological fluids.