Recombinant Human HYAL1 Protein (His Tag)

Specifications

Catalog number

PDMH100438-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human hyaluronidases (HYALs) are a group of five endo‑ beta ‑N‑acetyl‑hexosaminidases that include HYAL1, HYAL2, HYAL3, HYAL4, and SPAM1 (PH20) . While HYAL1, HYAL2 and SPAM1 are endo‑ beta ‑N‑acetyl‑glucosaminidase that are mainly active on hyaluronan with little activity on chondroitin sulfate, HYAL4 is mainly active on chondroitin sulfate (type C and D in particular) (6) . Hyaluronan and chondroitin sulfate are abundant extracellular matrix components that have numerous biological functions. The backbone of chondroitin sulfate composed of repeating units of ‑4GlcA beta 1‑3GalNAc beta 1‑ most closely resembles to that of hyaluronan composed of repeating units of ‑4GlcA beta 1‑3GlcNAc beta 1‑, which may partially explain the overlapping substrate specificity of most hyaluronidases. HYAL1 is a lysosomal hyaluronidase with optimal activity around pH 4.0. It is highly expressed in the liver, kidney and heart, and is the predominant hyaluronidase found in plasma. Defects in HYAL1 are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. Surprisingly, HYAL1 has been reported both as a tumor promoter and suppressor. The enzymatic activity of recombinant human HYAL1 was measured using 35S‑labeled hyaluronan as substrate.

Abbreviation

HYAL1

Synonyms

NAT; MPS; Lung carcinoma protein; HYAL1; HYAL-1; LUCA1; MPS9; NAT6; Hyaluronic Lase; Hyaluronidase-1; Hyaluronoglucosaminidase-1; LuCa-1; HYAL 1; Hyaluronidase 1; hyaluronoglucosaminidase 1; LuCa 1; Lung carcinoma protein 1

UniProt

Q12794

Accession Number

Q12794

Expression System

Mammalian

Tag

C-His

Sequence

Met1-Trp435

Field of Research

Cancer; Cell Biology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

47.7 kDa

Observed Molecular Weight

50-60 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human