Rubicon / Baron Recombinant Rabbit Monoclonal Antibody [PSH08-25]

About this product

Rubicon (run domain Beclin-1-interacting and cysteine-rich domain-containing protein) is a protein that in humans is encoded by the RUBCN gene. Rubicon is one of the few known negative regulators of autophagy, a cellular process that degrades unnecessary or damaged cellular components. Rubicon is recruited to its sites of action through interaction with the small GTPase Rab7, and impairs the autophagosome-lysosome fusion step of autophagy through inhibition of PI3KC3-C2 (class III phosphatidylinositol 3-kinase complex 2). Negative modulation of Rubicon is associated with reduction of aging and aging-associated diseases: knockout of Rubicon increases lifespan in roundworms and female fruit flies, and in mice decreases kidney fibrosis and α-synuclein accumulation. In addition to regulation of autophagy, Rubicon has been shown to be required for LC3-associated phagocytosis (LAP) and LC3-associated endocytosis (LANDO). Rubicon has also been shown to negatively regulate the innate immune response through direct interaction with multiple downstream regulatory molecules.

Specifications

Catalog number

HA722969

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Baron antibody Beclin 1 associated RUN domain containing protein antibody Beclin-1 associated RUN domain containing protein antibody hypothetical protein LOC9711 antibody Kiaa0226 antibody OTTHUMP00000208021 antibody OTTHUMP00000208023 antibody OTTHUMP00000208024 antibody RUBIC_HUMAN antibody Rubicon antibody RUN domain and cysteine rich domain containing, Beclin 1 interacting protein antibody Run domain Beclin 1 interacting and cystein rich containing protein antibody Run domain Beclin-1 interacting and cysteine-rich containing protein antibody RUN domain protein as Beclin 1 interacting and cysteine rich containing antibody

Abbreviation

Run domain Beclin-1-interacting and cysteine-rich domain-containing protein, Rubicon, Beclin-1 associated RUN domain containing protein (Baron), RUBCN, KIAA0226

Swiss Prot

Q92622 Human, Q80U62 Mouse,

Cellular Locus

Late endosome, Lysosome, Early endosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant protein within human Rubicon aa 1-350.

Isotype

IgG

Clone

PSH08-25

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB

Positive Control

Raji cell lysate, A549 cell lysate, NCI-H1299 cell lysate, HeLa cell lysate, 293T cell lysate, COS-1 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse spleen tissue lysate.

Concentration

1 mg/mL

Dilution

WB: 1:2,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 109 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes