OPA1 Recombinant Rabbit Monoclonal Antibody [PSH06-39]

About this product

This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. The OPA1 protein localizes to the inner mitochondrial membrane, where it regulates mitochondrial fusion and cristae structure. OPA1 mediates mitochondrial fusion in cooperation with mitofusins 1 and 2 and participates in cristae remodeling by the oligomerization of two L-OPA1 and one S-OPA1, which then interact with other protein complexes to alter cristae structure. Its cristae regulating function also contributes to its role in oxidative phosphorylation and apoptosis, as it is required to maintain mitochondrial activity during low-energy substrate availability. Moreover, stabilization of mitochondrial cristae by OPA1 protects against mitochondrial dysfunction, cytochrome c release, and reactive oxygen species production, thus preventing cell death. Mitochondrial SLC25A transporters can detect these low levels and stimulate OPA1 oligomerization, leading to tightening of the cristae, enhanced assembly of ATP synthase, and increased ATP production. Stress from an apoptotic response can interfere with OPA1 oligomerization and prevent mitochondrial fusion.

Specifications

Catalog number

HA722673

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Dynamin like 120 kDa protein antibody Dynamin like 120 kDa protein, mitochondrial antibody Dynamin-like 120 kDa protein, form S1 antibody FLJ12460 antibody Juvenile kjer type optic atrophy antibody KIAA0567 antibody KJER type antibody Large GTP binding protein antibody largeG antibody MGM1 antibody Mitochondrial dynamin like 120 kDa protein antibody Mitochondrial dynamin like GTPase antibody NPG antibody NTG antibody OAK antibody OPA 1 antibody opa1 antibody OPA1 gene antibody OPA1_HUMAN antibody Optic atrophy 1 (autosomal dominant) antibody OPTIC ATROPHY 1 antibody Optic atrophy 1 gene protein antibody Optic atrophy 1 homolog (human) antibody Optic atrophy protein 1 antibody Optic atrophy protein 1 homolog antibody

Abbreviation

OPA1, KIAA0567

Swiss Prot

O60313 Human, P58281 Mouse, Q2TA68 Rat

Cellular Locus

Mitochondrion inner membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human OPA1 aa 201-960.

Isotype

IgG

Clone

PSH06-39

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell, FC, IP

Positive Control

HeLa cell lysate, HeLa treated with 10μM CCCP for 30 minutes cell lysate, MCF7 cell lysate, LNCaP cell lysate, PANC-1 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Mouse liver tissue lysate, Rat brain tissue lysate, human kidney tissue, human skeletal muscle tissue, mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue, rat retina tissue, HeLa.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:100 ;FC: 1:1,000 ;IP: 1-2μg/sample

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 112 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes