Histone H2B (acetyl K20) Recombinant Rabbit Monoclonal Antibody [PS01-46]

About this product

Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and long N-terminal and C-terminal tails, H2B is involved with the structure of the nucleosomes. Histone H2B is a structural protein that helps organize eukaryotic DNA. It plays an important role in the biology of the nucleus where it is involved in the packaging and maintaining of chromosomes, regulation of transcription, and replication and repair of DNA. Histone H2B helps regulate chromatin structure and function through post-translational modifications and specialized histone variants. Acetylation and ubiquitination are examples of two post-translational modifications that affect the function of histone H2B in particular ways. Hyperacetylation of histone tails helps DNA-binding proteins access chromatin by weakening histone-DNA and nucleosome-nucleosome interactions. Furthermore, acetylation of a specific lysine residue binds to bromine-containing domains of certain transcription and chromatin regulatory proteins. This docking facilitates the recruitment of these proteins to the correct region of the chromosome. Ubiquitinated histone H2B is often found in regions of active transcription. Through the facilitation of chromatin remodeling, it stimulates transcriptional elongation and sets the stage for further modifications that regulate multiple elements of transcription.

Specifications

Catalog number

HA721285

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

GL105 antibody H2B GL105 antibody H2B histone family member O antibody H2B histone family member S antibody H2B.1 antibody H2B.1 B antibody H2B.b antibody H2B.c antibody H2B.d antibody H2B.e antibody H2B.f antibody H2B.j antibody H2B.q antibody H2B/b antibody H2B/c antibody H2B/d antibody H2B/e antibody H2B/f antibody H2B/j antibody H2B/o antibody H2B/q antibody H2BFB antibody H2BFC antibody H2BFD antibody H2BFE antibody H2BFF antibody H2BFJ antibody H2BFO antibody H2BFQ antibody H2BFS antibody H2BGL105 antibody H2BQ antibody HIRIP2 antibody HIST1H2BB antibody HIST1H2BD antibody HIST1H2BH antibody HIST1H2BL antibody HIST1H2BM antibody HIST1H2BN antibody HIST2H2BE antibody histone cluster 2 antibody Histone H2B antibody histone H2B GL105 antibody Histone H2B type 1 B antibody Histone H2B type 1 D antibody Histone H2B type 1 H antibody Histone H2B type 1 L antibody Histone H2B type 1 M antibody Histone H2B type 1 N antibody Histone H2B type 2 E antibody Histone H2B.q antibody histone protein antibody

Abbreviation

H2BC12

Swiss Prot

O60814 Human, P06899 Human, P23527 Human, P33778 Human, P57053 Human, P58876 Human, P62807 Human, Q16778 Human, Q5QNW6 Human, Q8N257 Human, Q93079 Human, Q99877 Human, Q99879 Human, Q99880 Human, P10854 Mouse, Q64475 Mouse, Q00729 Rat

Cellular Locus

Nucleus, Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide.

Isotype

IgG

Clone

PS01-46

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell, ChIP

Positive Control

Hela treated with 500 ng/ml TSA for 4 hours whole cell lysate, human colon carcinoma tissue, human skin tissue, mouse skin tissue, rat skin tissue, Hela, NIH/3T3.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:50 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 14 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes