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Product details

SARS-CoV-2 S protein RBD (K417N), His Tag (MALS verified)

Catalog number SPD-C52Hs-100ug

Discontinued

Price for SPD-C52Hs-100ug

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Size / count 100 µg

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About this product

SARS-CoV-2 S protein RBD (K417N), His Tag (SPD-C52Hs) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (K417N)).

Specifications

Catalog number
SPD-C52Hs-100ug
Size / count
100 µg
Dry ice
No
Hazardous
No
Discontinued
Yes
Background
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region
Arg 319 - Lys 537
Host
HEK 293
Target
Spike RBD
Conjugation
Unconjugated
Tag
C-10×His
Source
SARS-CoV-2
Stability
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized SARS-CoV-2 S protein RBD (K417N), His Tag (Cat. No. SPD-C52Hs) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.4-6 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
26.5 kDa
Shipping Conditions
Room Temperature
Storage Conditions
-20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 S protein RBD (K417N), His Tag (MALS verified)?

The catalog number for SARS-CoV-2 S protein RBD (K417N), His Tag (MALS verified) is SPD-C52Hs-100ug.

Is SARS-CoV-2 S protein RBD (K417N), His Tag (MALS verified) in stock?

SARS-CoV-2 S protein RBD (K417N), His Tag (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 S protein RBD (K417N), His Tag (MALS verified)?

SARS-CoV-2 S protein RBD (K417N), His Tag (MALS verified) is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 S protein RBD (K417N), His Tag (MALS verified)?

Estimated delivery time for SARS-CoV-2 S protein RBD (K417N), His Tag (MALS verified) is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 S protein RBD (K417N), His Tag (MALS verified) used for?

SARS-CoV-2 S protein RBD (K417N), His Tag (SPD-C52Hs) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (K417N)).

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

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