PD-L1 Recombinant Rabbit Monoclonal Antibody [PSH08-56]

About this product

Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. Programmed death-ligand 1 (PD-L1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the adaptive arm of immune systems during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the adaptive immune system reacts to antigens that are associated with immune system activation by exogenous or endogenous danger signals. In turn, clonal expansion of antigen-specific CD8+ T cells and/or CD4+ helper cells is propagated. The binding of PD-L1 to the inhibitory checkpoint molecule PD-1 transmits an inhibitory signal based on interaction with phosphatases (SHP-1 or SHP-2) via Immunoreceptor Tyrosine-Based Switch Motif (ITSM). This reduces the proliferation of antigen-specific T-cells in lymph nodes, while simultaneously reducing apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells) – further mediated by a lower regulation of the gene Bcl-2. PD-L1 is expressed on both hematopoietic and nonhematopoietic cells in tissues. However, the exact roles of PD-L1 on hematopoietic versus nonhematopoietic cells in modulating immune responses are unclear.

Specifications

Catalog number

HA723002

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

B7 H antibody B7 H1 antibody B7 homolog 1 antibody B7-H1 antibody B7H antibody B7H1 antibody CD 274 antibody CD-274 antibody CD274 antibody CD274 antigen antibody CD274 molecule antibody MGC142294 antibody MGC142296 antibody OTTHUMP00000021029 antibody PD L1 antibody PD-L1 antibody PD1L1_HUMAN antibody PDCD1 ligand 1 antibody PDCD1L1 antibody PDCD1LG1 antibody PDL 1 antibody PDL1 antibody Programmed cell death 1 ligand 1 antibody Programmed death ligand 1 antibody RGD1566211 antibody

Abbreviation

Programmed cell death 1, PD-L1

Swiss Prot

A8WEV0 Cat

Cellular Locus

Membrane.

Host

Rabbit

Species Reactivity

Cat

Immunogen

Recombinant protein within cat PD-L1 aa 1-232.

Isotype

IgG

Clone

PSH08-56

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, IF-Tissue

Positive Control

293T cells transfected with cat PD-L1.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IF-Cell: 1:5,000 ;IHC-P: 1:20,000 ;IF-Tissue: 1:5,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 26 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes