Recombinant Human ANGPTL4 Protein (hIgG1 Fc Tag)

Specifications

Catalog number

PDMH100444-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ANGPTL4, also known as ANGPTL2, is a protein with hypoxia-induced expression in endothelial cells. It contains 1 fibrinogen C-terminal domain and is expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. ANGPTL4 inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. It may act as a regulator of angiogenesis and modulate tumorigenesis. It inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. It may also exert a protective function on endothelial cells through an endocrine action. ANGPTL4 is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM) . The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.

Abbreviation

ANGPTL4

Synonyms

UNQ; ARP; PRO; PSEC; angiopoietin like; Secreted protein Bk; Angiopoietin like protein; Angiopoietin-like protein; Angiopoietin related protein; Angiopoietin-related protein; ANGPTL;425O18; ANGPTL4; ARP4; FIAF; HARP; HFARP; NL2; PGAR; TGQTL; UNQ171; pp1158;425O18-1; Angiopoietin-like protein 4; Angiopoietin-related protein 4; Farp; Fasting-induced adipose factor; Hepatic fibrinogen/angiopoietin-related protein; Ng27; Secreted protein Bk89; PSEC0166; angiopoietin like 4; Angiopoietin like protein 4; Angiopoietin related protein 4; PRO197

UniProt

Q9BY76

Accession Number

Q9BY76

Expression System

Mammalian

Tag

C-hIgG1 Fc

Sequence

Arg164-Ser406

Field of Research

Metabolism; Cardiovascular; Cancer; Signal Transduction

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

51.6 kDa

Observed Molecular Weight

58 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human