KDM2A Rabbit Polyclonal Antibody

About this product

Lysine-specific demethylase 2A (KDM2A) also known as F-box and leucine-rich repeat protein 11 (FBXL11) is an enzyme that in humans is encoded by the KDM2A gene. KDM2A is a member of the superfamily of alpha-ketoglutarate-dependent hydroxylases, which are non-haem iron-containing proteins. This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least 6 highly degenerated leucine-rich repeats. FBXL11/KDM2A is a histone H3 lysine 36 demethylase enzyme. The enzymatic activity of FBXL11/KDM2A relies on a conserved JmjC domain in the N-terminus of the protein that co-ordinates iron and alphaketoglutarate to catalyze demethylation via a hydroxylation based mechanism. It has recently been demonstrated that a ZF-CxxC DNA binding domain within FBXL11/KDM2A has the capacity to interact with non-methylated DNA and this domain targets FBXL11/KDM2A to CpG island regions of the genome where it specifically removes histone H3 lysine 36 methylation. This mechanism acts to create a chromatin environment at CpG islands that highlights these regulatory elements and differentiates them from non-regulatory regions in large complex mammalian genomes. In a study in mouse hepatocytes, this gene was shown to regulate hepatic gluconeogenesis.

Specifications

Catalog number

HA500515

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

[Histone-H3]-lysine-36 demethylase 1A antibody CXXC-type zinc finger protein 8 antibody CXXC8 antibody F box / LRR repeat protein 11 antibody F box and leucine rich repeat protein 11 antibody F box protein FBL7 antibody F-box and leucine-rich repeat protein 11 antibody F-box protein FBL7 antibody F-box protein Lilina antibody F-box/LRR-repeat protein 11 antibody FBL11 antibody FBL7 antibody FBXL11 antibody JHDM1A antibody JmjC domain-containing histone demethylation protein 1A antibody Jumonji C domain containing histone demethylase 1A antibody kdm2a antibody KDM2A_HUMAN antibody LILINA antibody Lysine (K) specific demethylase 2A antibody Lysine-specific demethylase 2A antibody

Abbreviation

KDM2A, CXXC8, FBL11

Swiss Prot

Q9Y2K7 Human, P59997 Mouse,

Cellular Locus

Nucleus, nucleoplasm, Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human KDM2A aa 381-580 / 1,162.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P

Positive Control

MCF-7 cell lysate, Jurkat cell lysate, PC-12 cell lysate, human brain tissue lysate, rat colon tissue, human stomach tissue, mouse colon tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:1,000

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 133 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No