Recombinant Mouse IL-13 Protein (His Tag)

Specifications

Catalog number

PDEM100328-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 13 (IL-13) is a single-chain glycosylated polypeptide, which belongs to the IL-13/IL-4 family. IL-13 protein is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 exerts its effects through a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains (IL-13 Rα1 and IL-13 Rα2) . As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses, and diseases. Also, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. IL-13 protein and antibody are more importantly implicated as a central mediator of immunoregulatory processes in various cell types.

Abbreviation

IL-13

Synonyms

BHR; Interleukin; T-Cell Activation Protein P; ALRH; BHR1; T-Cell Activation Protein P600; Interleukin-13; IL13; NC30; P600; Il-13

UniProt

P20109

Accession Number

P20109

Expression System

E.coli

Tag

C-His

Sequence

Ser26-Phe131

Field of Research

Immunology; Cancer

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 80% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

11.5 kDa

Observed Molecular Weight

30-35 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse