Recombinant Rat Lipocalin-2 Protein (GST Tag)

Specifications

Catalog number

PDER100144-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.

Abbreviation

Lipocalin-2

Synonyms

24p; LCN; Oncogene 24p; NGALlipocalin;25 kDa alpha-2-microglobulin-related subunit of MMP; MSFI;24p3; HNL; LCN2; Lipocalin-2; NGAL; NGALlipocalin-2; p25; Siderocalin; Uterocalin; Lipocalin2;25 kDa alpha-2-microglobulin-related subunit of MMP-9; lipocalin 2 (oncogene 24p3) ; lipocalin 2; migration-stimulating factor inhibitor; neutrophil gelatinase-associated lipocalin; Oncogene 24p3;25 kDa alpha 2 microglobulin related subunit of MMP9; Alpha 2 microglobulin related protein; HGNC:6526; Lcn 2; Migration stimulating factor inhibitor; Neutrophil Gelatinase Associated Lipocalin; siderocalin LCN2; SV40 induced 24P3 protein

UniProt

P30152

Accession Number

P30152

Expression System

E.coli

Tag

N-GST

Sequence

Gln21-Asn198

Field of Research

Cancer; Cardiovascular; Immunology; Neuroscience; Tags and Cell Markers

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

45.6 kDa

Observed Molecular Weight

50 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Rat