Recombinant Human TNFRSF14 Protein (hIgG1 Fc Tag)

Specifications

Catalog number

PDMH100449-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Herpesvirus entry mediator (HVEM), also referred to as TNFRSF14, TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a member of type I transmembrane protein belonging to the TNF-receptor superfamily. It is expressed on many immune cells, including T and B cells, NK cells, monocytes, and neutrophils. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling. However, recent studies have revealed that HVEM is also involved in the unique inhibitory signaling pathway for T cells through activating tyrosine phosphorylation of the immunoreceptor tyrosine-based inhibitory motif (ITIM) in B and T lymphocyte attenuator (BTLA) . HVEM provides a stimulatory signal following engagement with LIGHT (TNFSF14) on T cells. In contrast, it can also provide an inhibitory signal to T cells when it binds the B and T lymphocyte attenuator (BTLA), a ligand member of the Immunoglobulin (Ig) superfamily. Thus, HVEM may be viewed as a molecular switch, capable of facilitating both stimulatory and inhibitory cosignaling in T cells. Substantial evidence from both human disease and from experimental mouse models has indicated that dysregulation of the LIGHT-HVEM-BTLA cosignaling pathway can cause inflammation in the lung and in mucosal tissues.

Abbreviation

TNFRSF14

Synonyms

UNQ; PRO; Tumor Necrosis Factor Receptor-Like; Tumor Necrosis Factor Receptor Superfamily Member; Tnfrsf; CD270; Herpes Virus Entry Mediator A; Herpesvirus Entry Mediator A; TR2; Tumor Necrosis Factor Receptor Superfamily Member 14; Tumor Necrosis Factor Receptor-Like 2; HVEA; HVEM; TNFRSF14; UNQ329; PRO509

UniProt

Q92956-1

Accession Number

Q92956-1

Expression System

Mammalian

Tag

C-hIgG1 Fc

Sequence

Leu39-Val202

Field of Research

Immunology; Signal Transduction; Cancer; Microbiology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

42.9 kDa

Observed Molecular Weight

50-55 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human