Phospho-DNA PKcs (S2056) Recombinant Rabbit Monoclonal Antibody [JE41-92]

About this product

DNA-dependent protein kinase, catalytic subunit, also known as DNA-PKcs, is an enzyme that in humans is encoded by the gene designated as PRKDC or XRCC7. DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family. The DNA-Pkcs protein is a serine/threonine protein kinase consisting of a single polypeptide chain of 4,128 amino acids. DNA-PKcs is the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase called DNA-PK. The second component is the autoimmune antigen Ku. On its own, DNA-PKcs is inactive and relies on Ku to direct it to DNA ends and trigger its kinase activity. DNA-PKcs is required for the non-homologous end joining (NHEJ) pathway of DNA repair, which rejoins double-strand breaks. It is also required for V (D)J recombination, a process that utilizes NHEJ to promote immune system diversity. Many proteins have been identified as substrates for the kinase activity of DNA-PK. Autophosphorylation of DNA-PKcs appears to play a key role in NHEJ and is thought to induce a conformational change that allows end processing enzymes to access the ends of the double-strand break. DNA-PK also cooperates with ATR and ATM to phosphorylate proteins involved in the DNA damage checkpoint.

Specifications

Catalog number

HA722066

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DNA dependent protein kinase catalytic subunit antibody DNA PK catalytic subunit antibody DNA-dependent protein kinase catalytic subunit antibody DNA-PK catalytic subunit antibody DNA-PKcs antibody DNAPK antibody DNAPK catalytic subunit antibody DNPK 1 antibody DNPK1 antibody Hyper radiosensitivity of murine scid mutation, complementing 1 antibody Hyperradiosensitivity complementing 1, mouse, homolog of 1 antibody HYRC 1 antibody HYRC antibody HYRC1 antibody IMD26 antibody p350 antibody p460 antibody PKRDC antibody PRKDC antibody PRKDC_HUMAN antibody Protein Kinase DNA Activated Catalytic Polypeptide antibody XRCC 7 antibody XRCC7 antibody

Abbreviation

PRKDC, HYRC, HYRC1

Swiss Prot

P78527 Human

Cellular Locus

Nucleus, nucleolus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide corresponding to residues surrounding Ser2056 of human DNA-PKcs protein.

Isotype

IgG

Clone

JE41-92

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell

Positive Control

HT-29 treated with UV for 3 hours cell lysate, HT-29 cells treated with UV for 3 hours.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 469 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes