Phospho-p53 (S33) Recombinant Rabbit Monoclonal Antibody [JE41-97]

About this product

p53, also known as Tumor protein P53, cellular tumor antigen p53 (UniProt name), or transformation-related protein 53 (TRP53) is a regulatory protein that is often mutated in human cancers. The p53 proteins (originally thought to be, and often spoken of as, a single protein) are crucial in vertebrates, where they prevent cancer formation. As such, p53 has been described as "the guardian of the genome" because of its role in conserving stability by preventing genome mutation. Hence TP53 is classified as a tumor suppressor gene. The TP53 gene is the most frequently mutated gene (>50%) in human cancer, indicating that the TP53 gene plays a crucial role in preventing cancer formation. TP53 gene encodes proteins that bind to DNA and regulate gene expression to prevent mutations of the genome. In addition to the full-length protein, the human TP53 gene encodes at least 12 protein isoforms.

Specifications

Catalog number

HA722703

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Antigen NY-CO-13 antibody BCC7 antibody Cellular tumor antigen p53 antibody FLJ92943 antibody LFS1 antibody Mutant tumor protein 53 antibody p53 antibody p53 tumor suppressor antibody P53_HUMAN antibody Phosphoprotein p53 antibody Tp53 antibody Transformation related protein 53 antibody TRP53 antibody tumor antigen p55 antibody Tumor protein 53 antibody Tumor protein p53 antibody Tumor suppressor p53 antibody

Abbreviation

TP53, P53

Swiss Prot

P04637 Human, P02340 Mouse, P10361 Rat

Cellular Locus

Cytoplasm, Nucleus, PML body, Endoplasmic reticulum, Mitochondrion matrix, cytoskeleton, microtubule organizing center, centrosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser33 of Human p53 aa 1-50 / 393.

Isotype

IgG

Clone

JE41-97

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

HT-29 cell lysate, HT-29 treated with 100ng/mL Nocodazole for 18 hours cell lysate, HT-29 cells treated with 100ng/mL Nocodazole for 18 hours, mouse liver tissue, human breast cancer tissue, human colon cancer tissue, human colon tissue, rat liver tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100 ;IHC-P: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 53 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes