Myc tag, AlpHcAbs® Mouse IgG2a

About this product

Anti-Myc tag, AlpHcAbs® Mouse IgG2a antibody is designed for detecting Myc tag fusion protein specifically. Anti-Myc tag, AlpHcAbs® Mouse IgG2a antibody is recombinant fab of alpaca IgG1 fused to mouse IgG2a Fc. Based on western blot and ELISA, Anti-Myc tag, AlpHcAbs® Mouse IgG2a antibody reacts with the Myc tag sequence (EQKLISEEDL) selectively, no reactivity with other proteins. The Myc peptide are widely used for detecting or manipulating proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, Myc tag is unlikely to affect the tagged protein’s biochemical properties. Myc tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Using antibody with Fc (mutation), the background from Fc receptors will be eliminated.

Specifications

Catalog number

HA710206

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Host

Alpaca

Species Reactivity

Universal

Immunogen

Myc tag fusion protein

Conjugation

Non-conjugated

Type

Alpaca monoclonal Antibody

Applications

WB, ICC/IF, ELISA, IP, FC

Concentration

1 mg/mL

Dilution

WB: 1:10,000-1:50,000 ;ICC/IF: 1:200-1:1000 ;ELISA: 1:10,000-1:50,000 ;IP: 1-2ug/sample ;FC: 1µg for 106 cells ; ;Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Purity

Recombinant Expression and Affinity purified

Form

Liquid

Buffer

Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol

Storage Conditions

Store at –20°C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C