BAX Recombinant Rabbit Monoclonal Antibody [PD01-44]

About this product

Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that in humans is encoded by the BAX gene. BAX is a member of the Bcl-2 gene family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated. BAX is believed to interact with, and induce the opening of the mitochondrial voltage-dependent anion channel, VDAC. Alternatively, growing evidence also suggests that activated BAX and/or Bak form an oligomeric pore, MAC in the MOM (mitochondrial outer membrane). This results in the release of cytochrome c and other pro-apoptotic factors from the mitochondria, often referred to as mitochondrial outer membrane permeabilization, leading to activation of caspases. This defines a direct role for BAX in mitochondrial outer membrane permeabilization. BAX activation is stimulated by various abiotic factors, including heat, hydrogen peroxide, low or high pH, and mitochondrial membrane remodeling. In addition, it can become activated by binding BCL-2, as well as non-BCL-2 proteins such as p53 and Bif-1. Conversely, BAX can become inactivated by interacting with VDAC2, Pin1, and IBRDC2.

Specifications

Catalog number

HA721324

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Apoptosis regulator BAX antibody BAX antibody Bax-protein antibody BAX_HUMAN antibody BAXA antibody Baxdelta2G9 antibody Baxdelta2G9omega antibody Baxdelta2omega antibody Bcl-2-like protein 4 antibody BCL2 associated X protein antibody BCL2 associated X protein omega antibody BCL2 associated X protein transcript variant delta2 antibody Bcl2-L-4 antibody BCL2L4 antibody membrane isoform alpha antibody

Abbreviation

BAX, BCL2L4

Swiss Prot

Q07812 Human

Cellular Locus

Mitochondrion outer membrane, Cytoplasm.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human Bax aa 50-150 (internal sequence).

Isotype

IgG

Clone

PD01-44

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

IHC-P

Positive Control

Human breast tissue, human cervical carcinoma tissue, human kidney tissue.

Concentration

1 mg/mL

Dilution

IHC-P: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 21 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes