Glucokinase (GCK) Activity Colorimetric Assay Kit

Specifications

Catalog number

E-BC-K768-M

Size / count

96 T (40 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Type

Colorimetric method

Field of Research

Glycolysis and Lipid Metabolism; Metabolic Diseases

Assay Principle

Glucokinase (GCK) is a key enzyme in the glucose metabolism pathway. It participates in the first step of glycolysis, catalyzes glucose phosphorylation, promotes insulin secretion and glucose metabolism, and effectively controls blood glucose balance in internal. GCK, also known as hexokinase IV, is mainly expressed in hepatocytes and islet β-cell, and has a stronger affinity for glucose than other hexokinases. Therefore, the total hexokinase activity in the sample is measured at high glucose concentration, and the hexokinase activity with low glucose affinity is measured at low glucose concentration. The GCK activity in the sample can be obtained by subtracting the total hexokinase from the total hexokinase. The detection principle of this kit: GCK catalyzes the substrate reaction to produce NADPH, which reacts with the chromogenic agent. The activity of GCK can be calculated by measuring the generation rate of chromogenic substance at 450 nm.

Assay Performance Time

30 min

Sample Type

Animal tissue; Cell

Detection Range

2.62-42.80 U/L

Sensitivity

2.62 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

1.3-7.4%

Intra CV

2.9-6.6%

Detection Instrument

Microplate reader