PLK1 Recombinant Rabbit Monoclonal Antibody [PSH04-35]

About this product

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL. NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. Phosphorylation of NINL component of the centrosome leads to NINL dissociation from other centrosomal proteins. Involved in mitosis exit and cytokinesis by phosphorylating CEP55, ECT2, KIF20A/MKLP2, CENPU, PRC1 and RACGAP1. Recruited at the central spindle by phosphorylating and docking PRC1 and KIF20A/MKLP2; creates its own docking sites on PRC1 and KIF20A/MKLP2 by mediating phosphorylation of sites subsequently recognized by the POLO box domains. Phosphorylates RACGAP1, thereby creating a docking site for the Rho GTP exchange factor ECT2 that is essential for the cleavage furrow formation. Promotes the central spindle recruitment of ECT2. Plays a central role in G2/M transition of mitotic cell cycle by phosphorylating CCNB1, CDC25C, FOXM1, CENPU, PKMYT1/MYT1, PPP1R12A/MYPT1 and WEE1.

Specifications

Catalog number

HA722118

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Cell cycle regulated protein kinase antibody PLK 1 antibody PLK antibody PLK-1 antibody plk1 antibody PLK1_HUMAN antibody Polo like kinase 1 antibody Polo-like kinase 1 antibody Serine/threonine protein kinase 13 antibody Serine/threonine protein kinase PLK1 antibody Serine/threonine-protein kinase 13 antibody Serine/threonine-protein kinase PLK1 antibody STPK 13 antibody STPK13 antibody

Abbreviation

PLK1, PLK

Swiss Prot

P53350 Human, Q07832 Mouse, Q62673 Rat

Cellular Locus

Nucleus, Chromosome, centromere, kinetochore, Cytoplasm, cytoskeleton, microtubule organizing center, spindle, Midbody.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant protein within human PLK1 aa 314-603 / 603.

Isotype

IgG

Clone

PSH04-35

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

HeLa cell lysate, K-562 cell lysate, HEK-293 cell lysate, Raji cell lysate, HT-29 cell lysate, HCT 116 cell lysate, COS-1 cell lysate, RAW264.7 cell lysate, C6 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, HeLa, RAW264.7, PC-12, human colon cancer tissue, mouse colon tissue, rat colon tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100 ;IHC-P: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 68 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes