2xHieff™ Ultra-Rapid II HotStart PCR Master Mix

About this product

2×Hieff™ Ultra-Rapid II HotStart PCR Master Mix contains heat-stabilized Taq DNA Polymerase modified with antibodies, adds strong elongation factor and optimized buffer system, and has super high amplification efficiency. It is very suitable for PCR amplification of most colonies such as E. coli, Agrobacterium, and yeast. The extension speed of amplification of complex templates within 3 kb can reach 1 sec/kb, 3-6 kb to 3 sec/kb, 6-10 kb to 5 sec/kb and 10 kb to 10 sec/kb; the amplification speed of simple templates such as plasmid within 6 kb can reach 1 sec/kb, which can greatly save PCR reaction time. Meanwhile, Mix contains dNTPs, Mg2+, and it can be amplified only with adding primers and template. In addition, Mix contains red tracer dye, which can be used by electrophoresis directly after the end of the reaction. The protective agent added to the system enables the product to maintain a stable activity after repeated freezing and thawing. The 3′ end of the PCR product bands A and can be easily cloned into the T vector.

Specifications

Catalog number

10167ES03

Size / count

1 mL

Dry ice

Yes

Hazardous

No

Discontinued

No

Specifications

<div style="text-align: justify;"><strong data-start="2107" data-end="2159">2× Hieff™ Ultra-Rapid II HotStart PCR Master Mix</strong> is supplied as a <strong data-start="2177" data-end="2211">2× concentrated PCR master mix</strong> containing antibody-modified thermostable Taq DNA polymerase, dNTPs, Mg²⁺ and optimized reaction components. The product is designed for rapid DNA amplification and supports applications including colony PCR, rapid PCR, genotype identification and amplification of complex templates. It reports amplification capabilities for targets with <strong data-start="2555" data-end="2612">GC content up to 70% and fragment lengths up to 10 kb</strong> in its product positioning, with rapid extension performance reported at up to <strong data-start="2692" data-end="2704">1 sec/kb</strong> under applicable conditions. The product is supplied in <strong data-start="2761" data-end="2790">1 mL and 5 × 1 mL formats</strong> and is stored at <strong data-start="2841" data-end="2869">−25 to −15°C for 2 years</strong>.</div>

Field of Research

Molecular Biology

Features & Benefits

<ul data-start="2959" data-end="3548"><li data-section-id="1mk44f3" data-start="2959" data-end="2995"><strong data-start="2961" data-end="2995">2× ready-to-use PCR master mix</strong><span aria-hidden="true" class="PDq2pG_selectionAnchor"></span> </li><li data-section-id="rw0wju" data-start="2996" data-end="3051">Antibody-modified thermostable <strong data-start="3029" data-end="3051">Taq DNA polymerase</strong> </li><li data-section-id="1oxmkhb" data-start="3052" data-end="3077">Strong extension factor </li><li data-section-id="2pbz4h" data-start="3078" data-end="3107">Optimized PCR buffer system </li><li data-section-id="vnxpn2" data-start="3108" data-end="3129">Rapid amplification </li><li data-section-id="e7rn8b" data-start="3130" data-end="3155">Suitable for colony PCR </li><li data-section-id="1k6v7bn" data-start="3156" data-end="3194">Suitable for genotype identification </li><li data-section-id="181uiox" data-start="3195" data-end="3227">Suitable for complex templates </li><li data-section-id="n2vat0" data-start="3228" data-end="3308">Supports high-GC templates up to <strong data-start="3263" data-end="3270">70%</strong> according to manufacturer information </li><li data-section-id="rmb7e0" data-start="3309" data-end="3389">Target amplification up to <strong data-start="3338" data-end="3347">10 kb</strong> in the manufacturer's product positioning </li><li data-section-id="1314hpz" data-start="3390" data-end="3469">Rapid extension performance of up to <strong data-start="3429" data-end="3441">1 sec/kb</strong> under applicable conditions </li><li data-section-id="1ia2wyb" data-start="3470" data-end="3548">Stable for <strong data-start="3483" data-end="3510">2 years at −25 to −15°C</strong> <span class="contents" data-content-reference-start="3473" data-content-reference-end="3505"><span class="" data-state="closed"></span></span> </li> </ul>

Shipping Conditions

Dry ice

Package Size

1 mL