p53 (acetyl K382) Recombinant Rabbit Monoclonal Antibody [PSH06-88]

Catalog number HA722763

Price for HA722763

Size / count 100 µL

About this product

p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

Specifications

Catalog number: HA722763
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: Antigen NY-CO-13 antibody BCC7 antibody Cellular tumor antigen p53 antibody FLJ92943 antibody LFS1 antibody Mutant tumor protein 53 antibody p53 antibody p53 tumor suppressor antibody P53_HUMAN antibody Phosphoprotein p53 antibody Tp53 antibody Transformation related protein 53 antibody TRP53 antibody tumor antigen p55 antibody Tumor protein 53 antibody Tumor protein p53 antibody Tumor suppressor p53 antibody
Abbreviation: TP53, P53
Swiss Prot: P04637 Human
Cellular Locus: Cytoplasm, Nucleus, Nucleus, PML body, Endoplasmic reticulum, Mitochondrion matrix, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Host: Rabbit
Species Reactivity: Human
Immunogen: Synthetic peptide within human p53 (acetyl K382) aa 344-393.
Isotype: IgG
Clone: PSH06-88
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell
Positive Control: HepG2 starved overnight treated with 30μg/mL etoposide for 7 hours then add 500ng/mL TSA for 3 hours cell lysate, HeLa cells treated with 500ng/mL TSA for 4 hours.
Concentration: 1 mg/mL
Dilution: WB: 1:1,000 ;IF-Cell: 1:200
Purity: Protein A affinity purified.
Form: Liquid
Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 53 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for p53 (acetyl K382) Recombinant Rabbit Monoclonal Antibody [PSH06-88]?

The catalog number for p53 (acetyl K382) Recombinant Rabbit Monoclonal Antibody [PSH06-88] is HA722763.

What sizes are available for p53 (acetyl K382) Recombinant Rabbit Monoclonal Antibody [PSH06-88]?

p53 (acetyl K382) Recombinant Rabbit Monoclonal Antibody [PSH06-88] is available in the following size(s): 100 µL.

Is p53 (acetyl K382) Recombinant Rabbit Monoclonal Antibody [PSH06-88] in stock?

p53 (acetyl K382) Recombinant Rabbit Monoclonal Antibody [PSH06-88] is currently made to order / not held in immediate stock. Contact us for lead time.

What is p53 (acetyl K382) Recombinant Rabbit Monoclonal Antibody [PSH06-88] used for?

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.