SARS-CoV-2 Spike NTD Protein (C136F, Y144del, R190S, D215G, LA242-243del), His Tag (MALS verified)

About this product

SARS-CoV-2 Spike NTD, His Tag (SPD-C5229) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1). The mutations C136F, Y144del, R190S, D215G, LA242-243del were identified in the SARS-CoV-2 variant C.1.2.

Specifications

Catalog number

SPD-C5229-1mg

Size / count

1 mg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 34.5 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Ser 13 - Leu 303

Host

HEK 293

Target

Spike NTD

Conjugation

Unconjugated

Tag

C-10×His

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized SARS-CoV-2 Spike NTD, His Tag (Cat. No. SPD-C5229) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb (Cat. No. SPD-M121) with a linear range of 0.1-2 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

34.5 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C