PCB Recombinant Rabbit Monoclonal Antibody [PSH01-55]

About this product

Pyruvate carboxylase (PC) encoded by the gene PC is an enzyme (EC 6.4.1.1) of the ligase class that catalyzes (depending on the species) the physiologically irreversible[citation needed] carboxylation of pyruvate to form oxaloacetate (OAA). During gluconeogenesis, pyruvate carboxylase is involved in the synthesis of phosphoenolpyruvate (PEP) from pyruvate. Pyruvate is first converted by pyruvate carboxylase to oxaloacetate (OAA) in the mitochondrion requiring hydrolysis of one molecule of ATP. The OAA is then decarboxylated and simultaneously phosphorylated, which is catalyzed by one of two isoforms of phosphoenolpyruvate carboxykinase (PEPCK) either in the cytosol or in the mitochondria to produce PEP. Under ordinary gluconeogenic conditions, OAA is converted into PEP by mitochondrial PEPCK; the resultant PEP is then transported out of the mitochondrial matrix by an anion transporter carrier system, and converted into glucose by cytosolic gluconeogenic enzymes. However, during starvation when cytosolic NADH concentration is low and mitochrondrial NADH levels are high oxaloacetate can be used as a shuttle of reducing equivalents. As such OAA is converted into malate by mitochondrial malate dehydrogenase (MDH). After export into the cytosol, malate is converted back into OAA, with concomitant reduction of NAD+; OAA is subsequently converted to PEP which is available for gluconeogenesis in the cytosol along with the transported reducing equivalent NADH.

Specifications

Catalog number

HA721698

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

mitochondrial antibody OTTHUMP00000235155 antibody OTTHUMP00000235156 antibody PC antibody PCB antibody Pcx antibody PYC_HUMAN antibody Pyruvate carboxylase antibody Pyruvate carboxylase mitochondrial antibody Pyruvic carboxylase antibody

Abbreviation

Pyruvate carboxylase, mitochondrial Pyruvic carboxylase (PCB) PC

Swiss Prot

P11498 Human, Q05920 Mouse, P52873 Rat

Cellular Locus

Mitochondrion matrix.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human PCB aa 450-1178.

Isotype

IgG

Clone

PSH01-55

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell, FC, IP

Positive Control

HepG2 cell lysate, MCF7 cell lysate, A549 cell lysate, Huh7 cell lysate, Hela cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, mouse liver tissue, mouse lymph node tissue, rat liver tissue, rat brain tissue, HepG2, PC-12.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:200 ;IF-Cell: 1:250 ;FC: 1:1,000 ;IP: 1-2μg/sample

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 130 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes