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Product details

Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit

Catalog number E-UNEL-M0167-01

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Price for E-UNEL-M0167-01

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Size / count 5x 96 Tests

Specifications

Catalog number
E-UNEL-M0167-01
Size / count
5x 96 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
PDGF-BB
UniProt
P31240
Cross Reactivity
This kit recognizes PDGF-BB in samples.No significant cross-reactivity or interference between PDGF-BB and analogues was observed.
Target
C-sis; IBGC5; PDGF2; PDGF-2; PDGFB; Platelet-derived growth factor subunit B; SIS; SSV; PDGFBB; PDGF-BB
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cell Biology; Immunology; Neuroscience; Stem Cells
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PDGF-BB . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PDGF-BB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PDGF-BB , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PDGF-BB . You can calculate the concentration of PDGF-BB in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
24 h
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%
Recovery
80%-120%
Shipping Conditions
Ice bag
Storage Conditions
-20°C,12 months
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse PDGF-BB concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit?

The catalog number for Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit is E-UNEL-M0167.

What sizes are available for Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit?

Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit is available in the following size(s): 5x 96 Tests, 15x 96 Tests.

How many pack sizes does Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit come in?

Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit is available in 2 different pack sizes.

Is Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit in stock?

Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit?

Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit?

Estimated delivery time for Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit?

The safety data sheet for Uncoated Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit is available here: https://789.bio/eb/qS8Rh1.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

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