TRBP Recombinant Rabbit Monoclonal Antibody [JE34-99]

About this product

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR. Mediates recruitment of FTSJ3 methyltransferase to HIV-1 RNA, leading to 2'-O-methylation of the viral genome, allowing HIV-1 to escape the innate immune system.

Specifications

Catalog number

HA721922

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

RISC-loading complex subunit TARBP2 antibody TAR RNA-binding protein 2 antibody Trans-activation-responsive RNA-binding protein antibody TARBP2 antibody TRBP antibody

Abbreviation

RISC-loading complex subunit TARBP2 TAR RNA-binding protein 2 Trans-activation-responsive RNA-binding protein TARBP2 TRBP

Swiss Prot

Q15633 Human, P97473 Mouse, Q3SWU0 Rat

Cellular Locus

Cytoplasm. Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human TRBP aa 201-300 / 366.

Isotype

IgG

Clone

JE34-99

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell, FC

Positive Control

HeLa cell lysate, MCF7 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, A549 cell lysate, mouse testis tissue lysate, mouse liver tissue, rat liver tissue, MCF7.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:500-1:2,000 ;IF-Cell: 1:100 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 39 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes